Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Advancement in statistical modelling of chemical impurity profiles in forensic science.

Talanta·2026

Same author

Analysis of ligand recognition by choline O-acetyltransferase reveals thiol-reactive assay interference and weak ligand affinity in solution.

The Journal of biological chemistry·2026

Same author

Correction: Correlative X-ray Imaging to Reveal the Dissolution of Nanoparticles and Nutrient Transport in Plant Foliar Fertilization.

Frontiers in plant science·2026

Same author

Synchrotron X-rays elucidate the intracellular storage of the iodide and bromide antioxidant system in the kelp Laminaria digitata.

Journal of the Royal Society, Interface·2026

Same author

Distinct Foliar Uptake Pathways for Phosphorus and Nano-Hydroxyapatite in Potato Revealed By Synchrotron μCT and ³³P Imaging.

Plant, cell & environment·2026

Same author

Quantifying Trace Metals in Gunflint Microfossils by 3D Correlative X-ray Nanoimaging.

Analytical chemistry·2026

Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026

Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026

Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026

Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026

Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026

Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 29, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Single-distance nano-holotomography with coded apertures.

Viktor Nikitin, Marcus Carlsson, Rajmund Mokso

|January 16, 2025

Summary

This summary is machine-generated.

This study introduces a new single-distance method for high-resolution 3D imaging, improving temporal resolution for dynamic experiments. It uses coded apertures and joint reconstruction to overcome limitations of traditional multi-distance nano-holotomography.

More Related Videos

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Published on: January 12, 2013

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Published on: September 16, 2022

Related Experiment Videos

Last Updated: Apr 29, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Published on: January 12, 2013

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Published on: September 16, 2022

Area of Science:

Advanced imaging techniques
X-ray microscopy
Phase-contrast imaging

Background:

High-resolution 3D imaging via nano-holotomography traditionally requires multiple sample-to-detector distances.
This multi-distance approach limits temporal resolution, hindering operando studies.
Sample shifting introduces reconstruction complexities, including alignment and magnification correction.

Purpose of the Study:

To develop and validate a novel single-distance approach for phase-contrast 3D imaging.
To overcome the temporal resolution limitations of traditional multi-distance methods.
To enable artifact-free reconstructions for dynamic experiments.

Main Methods:

Utilizing coded apertures to structure beam illumination during sample rotation.
Implementing a joint reconstruction scheme integrating phase retrieval with 3D tomography.
Validating the approach through simulations.

Main Results:

Demonstrated a feasible single-distance method for nano-holotomography.
Achieved artifact-free reconstructions from a single distance.
Significantly improved temporal resolution compared to multi-distance techniques.

Conclusions:

The proposed single-distance method enhances temporal resolution for 3D imaging.
This technique is suitable for dynamic operando studies.
Coded apertures and joint reconstruction offer a promising alternative for advanced tomography.