Related Experiment Video
Updated: Jun 1, 2025
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
Published on: October 18, 2010
Polyphenol-modified 3D Nanoassemblies: A novel antibacterial immunoglobulins loading platform for rapid detection of
Di Yang1, Jianhang Zhou1, Shaochi Wang1
1College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road Yangling, 712100, Shaanxi, China.
Abstract:
Within the realm of lateral flow assay (LFIA), the conjugation efficiency between signal tracers and antibody constitutes a pivotal determinant for the sensitivity of the detection system. In this study, three-dimensional (3D) complex flower-like MoS2 self-assembled from 2D MoS2, and natural plant polyphenols "Tannic acid" were introduced for surface modification. This composite material exhibits distinct colorimetric signals, excellent monoclonal antibody coupling efficiency, and commendable photothermal properties.Finally, it was used as a signal tracer to establish a 12-min colorimetric/photothermal dual-mode LFIA platform (MoS2/TA-LFIA) for Salmonella Typhimurium detection. Compared to the AuNPs-based LFIA, the dual-mode detection platform limits are decreased by 20-fold (to 5 × 103 cfu/mL) and 100-fold (to 103 cfu/mL), respectively. Furthermore, the MoS2/TA-LFIA exhibits satisfactory recovery and stability when applied to milk and orange juice samples. Thus, this study provides a novel, efficient and reliable antibody loading strategy for LFIA detection of foodborne pathogens.
