Entropy driven-based catalytic biosensors for bioanalysis: From construction to application-A review

Area of Science:

• Biomedical Engineering
• Molecular Diagnostics
• Nanotechnology

Background:

• Precision medicine and emerging pathogens demand advanced biosensor capabilities.
• Current amplification methods in biosensors face trade-offs between sensitivity, selectivity, and operational complexity.
• Enzyme-based methods are sensitive but complex; enzyme-free methods are simple but often lack sensitivity and selectivity.

Purpose of the Study:

• To review the advantages of entropy-driven catalyst (EDC) biosensors.
• To explore the construction of DNA nanomachines using EDC for enhanced biosensing.
• To discuss applications in intracellular detection, multiplexed detection, and point-of-care testing (POCT).

Main Methods:

• Summarizing key advantages of EDC-based biosensing.
• Reviewing strategies for constructing DNA nanomachines for specific detection tasks.
• Analyzing the potential of EDC biosensors for clinical diagnosis and treatment.

Main Results:

• EDC biosensors achieve high sensitivity and selectivity concurrently.
• EDC biosensors offer operational simplicity and construction flexibility.
• EDC technology enables intracellular and multiplexed target detection.

Conclusions:

• EDC biosensors present a promising solution to current biosensor limitations.
• Applications in POCT can address practical clinical needs.
• EDC sensors are poised to become a significant area in biosensor development.