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Differential DNA Extraction from Lateral Flow Immunochromatographic Tests via the EZ1® Advanced XL System
Scarlet Neilson1, Leah Nangeroni2, Mirna Ghemrawi2
1Department of Chemistry & Physics, Arcadia University, 450 S Easton Rd, Glenside, PA 19038, USA.
Methods and Protocols
|January 23, 2025
Summary
This study presents a DNA extraction protocol for lateral flow immunochromatographic (LFI) test sample pads. The method successfully isolates male DNA profiles from mixed biological samples, aiding forensic investigations.
Area of Science:
- Forensic Science
- Molecular Biology
- Genetics
Background:
- Lateral flow immunochromatographic (LFI) tests are common in forensic analysis.
- Isolating DNA from LFI test sample pads, especially from mixed biological samples, presents challenges.
Purpose of the Study:
- To develop and validate a differential extraction protocol for isolating DNA from LFI test sample pads.
- To enable the separation of sperm and non-sperm fractions for targeted DNA analysis.
Main Methods:
- A two-step sequential lysis procedure was applied to sample pads from LFI tests.
- Differential extraction was used to separate non-sperm and sperm fractions.
- DNA was analyzed for autosomal and Y-STR profiles.
Main Results:
- Successful recovery of male autosomal and Y-STR DNA profiles was achieved.
- The protocol was effective even in mixed samples with a high proportion of female DNA.
- Demonstrated efficient DNA recovery from limited sample quantities on LFI test pads.
Conclusions:
- This differential extraction protocol provides an effective method for DNA recovery from LFI test sample pads.
- The method is valuable for forensic practitioners dealing with challenging mixed biological samples.
- Enhances the utility of LFI test pads in forensic DNA analysis.
