Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Novel 3D-Printed Flow Cell Design for <i>In Operando</i> Disposable Printed Electrode Replacement: Improving Continuous Methylene Blue Determination.

Micromachines·2026
Same author

Dimerized Power: The Antimicrobial and Antiviral Promise of Biflavonoids.

Biomolecules·2026
Same author

Aging Stability and Radical Activity of Plasma-Activated Water Treated in Liquid- and Gas-Phase Reactors.

Molecules (Basel, Switzerland)·2025
Same author

Development of Sustainable Biocatalytic Furfurylamine Production in a Magnetic Field-Assisted Microfluidic Reactor.

ACS sustainable chemistry & engineering·2025
Same author

Valorization of Food Waste: Extracting Bioactive Compounds for Sustainable Health and Environmental Solutions.

Antioxidants (Basel, Switzerland)·2025
Same author

Flow Synthesis of Pharmaceutical Intermediate Catalyzed by Immobilized DERA: Comparison of Different Immobilization Techniques and Reactor Designs.

Molecules (Basel, Switzerland)·2025

Related Experiment Video

Updated: May 30, 2025

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids
06:45

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids

Published on: August 9, 2024

1.0K

Development of an integrated micro-millisystem for continuous laccase extraction.

Marko Božinović1, Ivan Karlo Cingesar1, Domagoj Vrsaljko1

  • 1University of Zagreb Faculty of Chemical Engineering and Technology, Marulićev trg 19, HR-10000 Zagreb, Croatia.

Bioresource Technology
|January 25, 2025
PubMed
Summary

This study enhances enzyme extraction using deep eutectic solvents (DES) in aqueous two-phase systems (ATPS). The optimized process efficiently extracts laccase for superior aniline dye degradation, promoting sustainable biocatalysis.

Keywords:
ATPSDESDye removalExtractionLaccaseMicrosystems

More Related Videos

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes
09:51

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

9.0K
Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues
09:22

Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues

Published on: March 9, 2021

6.5K

Related Experiment Videos

Last Updated: May 30, 2025

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids
06:45

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids

Published on: August 9, 2024

1.0K
Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes
09:51

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

9.0K
Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues
09:22

Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues

Published on: March 9, 2021

6.5K

Area of Science:

  • Biocatalysis and Environmental Chemistry
  • Enzyme Technology and Green Chemistry

Background:

  • Growing environmental concerns drive the shift from chemical processes to enzymatic alternatives.
  • Laccases are versatile enzymes with significant potential for substrate oxidation and environmental applications.
  • Deep eutectic solvents (DES) offer tunable properties for improved biocatalytic reaction media.

Purpose of the Study:

  • To optimize deep eutectic solvent-based aqueous two-phase systems (DES-ATPS) for continuous laccase extraction.
  • To enhance the efficiency of biocatalytic reactions using extracted laccase.
  • To demonstrate the application of laccase in environmental remediation, specifically aniline dye degradation.

Main Methods:

  • Optimization of DES-ATPS for laccase extraction in a batch system.
  • Intensification of continuous laccase extraction using a microextractor setup.
  • Integration of a milliseparator for efficient continuous phase separation.
  • Application of extracted laccase as a co-solvent for aniline dye degradation.

Main Results:

  • Successful continuous extraction of laccase from crude samples using optimized DES-ATPS.
  • Significant increase in process efficiency through continuous extraction and phase separation.
  • Demonstrated superior efficiency of extracted laccase in degrading aniline dyes.
  • Validated the role of laccase as a co-solvent in enhancing biocatalytic degradation.

Conclusions:

  • DES-ATPS provide an effective and efficient method for continuous enzyme extraction.
  • Optimized laccase extraction advances enzyme recovery and application in biocatalysis.
  • Laccase exhibits significant potential for sustainable environmental remediation, particularly dye degradation.