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Isolating Free Carbenes, their Mixed Dimers and Organic Radicals
Published on: April 19, 2019
A new diterpenoid, carneadiol, isolated from Nocardia carnea IFM 12324
Yasumasa Hara1,2, Ayaka Nakamura3, Teruhisa Manome3
1Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki, Kagawa, 761-0795, Japan. hara.yasumasa@kagawa-u.ac.jp.
Abstract:
A new diterpenoid, carneadiol (1), with an unprecedented tricyclic carbon skeleton, was isolated from the culture extracts of Nocardia carnea IFM 12324. The structure of compound 1 was elucidated using spectral studies, including various NMR data. The absolute configuration of 1 was determined using X-ray crystallographic analysis with the crystalline sponge method. The biosynthetic gene of compound 1 was deduced, and it was observed that the mRNA of the gene involved in terpenoid cyclization increased significantly in the strain producing compound 1.
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