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Updated: May 30, 2025
Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
Published on: September 17, 2017
Highly sensitive fluorescent aptasensor based on magnetic metal-organic framework for aflatoxin B1 detection
Yanfei He1, Dongdong Zhang1, Qiong Wu1
1Engineering Research Center of Grain Storage and Security of Ministry of Education, Henan Provincial Engineering Technology Research Center on Grain Post Harvest, School of Food and Strategic Reserves, Henan University of Technology, Lianhua Road 100, Zhengzhou High-Tech Development Zone, Zhengzhou, 450001, Henan, China.
A novel magnetron fluorescence aptasensor was developed for sensitive aflatoxin B1 detection. This method offers a highly accurate and practical solution for ensuring food safety and protecting human health from this toxic compound.
Area of Science:
- Analytical Chemistry
- Biosensing
- Food Safety
Background:
- Aflatoxin B1 (AFB1) poses significant health risks due to its carcinogenicity, mutagenicity, and teratogenicity, necessitating sensitive detection methods.
- Existing detection techniques may lack the required sensitivity or be prone to background interference, impacting food safety assessments.
Purpose of the Study:
- To develop a highly sensitive and selective magnetron fluorescence aptasensor for the detection of Aflatoxin B1 (AFB1).
- To utilize Förster Resonance Energy Transfer (FRET) for enhanced detection efficiency and reduced background noise.
Main Methods:
- Conjugation of cadmium telluride (CdTe) quantum dots with AFB1 aptamers as fluorescent probes.
- Employing Fe3O4@UiO-66-NH2 nanocomplexes as magnetic carriers and fluorescence quenchers to improve signal-to-noise ratio.
- Development of a magnetron fluorescence aptasensor system integrating these components for FRET-based detection.
Main Results:
- The aptasensor achieved a linear detection range of 0.001-200 ng/mL for AFB1, with a low detection limit of 0.012 ng/mL.
- Demonstrated high selectivity, reproducibility, and stability, with excellent recoveries (95%-103%) in real corn and wheat samples.
- Results were validated against high-performance liquid chromatography (HPLC), confirming the aptasensor's accuracy.
Conclusions:
- The developed magnetron fluorescence aptasensor offers a highly sensitive, selective, and practical method for AFB1 detection.
- The sensor shows significant potential for routine application in food safety monitoring and public health protection.
- The integration of magnetic carriers and FRET significantly enhances detection performance.
