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    This study introduces a novel optical reflection method to visualize pure phase objects, overcoming limitations of intensity measurements. The technique enhances contrast and enables phase reconstruction for bioimaging applications.

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    Area of Science:

    • Optics
    • Photonics
    • Image Processing

    Background:

    • Phase distributions offer richer sample information than intensity distributions.
    • Pure phase objects are difficult to visualize due to weak scattering and absorption, limiting intensity measurements.
    • Existing methods struggle to reveal phase structure against background light.

    Purpose of the Study:

    • To develop a high-contrast method for visualizing pure phase objects.
    • To enable phase reconstruction for low-contrast phase objects.
    • To explore applications in bioimaging.

    Main Methods:

    • Utilizing optical reflection at a glass interface.
    • Exploiting light's spin-orbit interaction and the Brewster effect.
    • Performing a two-dimensional differentiation on the input light field.
    • Introducing bias retardation for phase reconstruction.

    Main Results:

    • Achieved high-contrast, isotropic differential images for pure phase objects.
    • Verified method superiority using phase masks with varying phase jumps.
    • Demonstrated phase reconstruction capability for low-contrast objects.

    Conclusions:

    • The proposed method effectively visualizes pure phase objects with enhanced contrast.
    • The technique offers a promising approach for bioimaging and other applications.
    • This method advances the ability to extract phase information from optical fields.