Biofilm formation by the plant growth promoting bacterium Bacillus cereus (EB-40)

The objective of this work was to investigate the biofilm production capacity of the isolate EB-40 (Bacillus cereus) in a culture medium for the multiplication of microorganisms and in roots of in vitro grown banana explants. It was observed that the isolate was able to produce biofilms in tryptone, soy and agar (TSA) culture medium and in the roots of explants. The format, architecture and location of the biofilms in TSA culture medium presented an exopolymer matrix formed by EB-40 presented coccoid bacillary cells and fibrillar structures. In roots explants was verified the formation of microcolonies adhered to the root hairs. The information obtained in this experiment allowed inferring the ability of the isolate to produce biofilms and the colonization pattern shown when associated with banana tree roots.