Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative colorimetric method for sulfamethazine in swine feeds.

D P Schwartz

    Journal - Association of Official Analytical Chemists
    |March 1, 1985
    PubMed
    Summary

    This study presents a simple colorimetric method for quantifying Sulfamethazine (SM) in animal feed. The developed assay offers accurate and precise results for SM detection, aiding in feed safety analysis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Analytical(13)C NMR: Detection, quantitation, and positional analysis of butyrate in butter oil.

    Lipids·2016
    Same author

    Formation of oxo- and hydroxyfatty acids in irradiated chickens.

    Meat science·2011
    Same author

    Non-supervised spatio-temporal analysis of interictal magnetic spikes: comparison with intracerebral recordings.

    Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology·2003
    Same author

    Spatiotemporal brain imaging of visual-evoked activity using interleaved EEG and fMRI recordings.

    NeuroImage·2001
    Same author

    Effects of SU101 in combination with cytotoxic agents on the growth of subcutaneous tumor xenografts.

    Clinical cancer research : an official journal of the American Association for Cancer Research·2000
    Same author

    Evaluation of a new MEG-EEG spatio-temporal localization approach using a realistic source model.

    Brain topography·1999

    Area of Science:

    • Analytical Chemistry
    • Food Science
    • Veterinary Pharmacology

    Background:

    • Accurate quantification of Sulfamethazine (SM) in animal feed is crucial for ensuring food safety and compliance with regulatory standards.
    • Existing methods for SM detection may be complex or lack the necessary throughput for routine analysis.

    Purpose of the Study:

    • To develop and validate a simple, accurate, and precise colorimetric method for the quantification of Sulfamethazine (SM) in swine feeds.
    • To establish a cost-effective analytical procedure suitable for high-volume sample analysis.

    Main Methods:

    • Isolation of SM from feed extracts using anion-exchange chromatography at pH 7.9.
    • Interference removal via pre-columns.
    • Diazotization and coupling of SM with Bratton-Marshall reagents.
    • Spectrophotometric determination of color intensity at 540 nm.
    • Development of a thin-layer chromatography system for SM verification.

    Main Results:

    • The colorimetric method accurately quantifies SM in feeds within the 1 to 130 ppm range with high precision.
    • The method demonstrates satisfactory specificity, is inexpensive, and allows for a high sample throughput.
    • A safe and simple method for preparing N1-methyl SM and its TLC verification was established.

    Conclusions:

    • The developed colorimetric method provides a reliable and efficient means for routine Sulfamethazine analysis in swine feed.
    • This assay contributes to enhanced feed safety monitoring and quality control.
    • The method's simplicity and cost-effectiveness make it suitable for widespread application in analytical laboratories.

    Related Experiment Videos