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Related Experiment Video

Updated: May 29, 2025

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Mitochondria-Activated Wash-Free Fluorescent Probe for Visualizing Single-Cell Photodamage.

Shuhuai Shen1, Danyang Hong1, Xuhong Qian1

  • 1Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China.

Analytical Chemistry
|February 3, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed Rhodalive, a new fluorescent probe for assessing cell viability. This mitochondria-targeted probe is activated by electron transport chain activity, offering wash-free imaging and reliable cell health assessment.

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Area of Science:

  • Biomedical research
  • Cell biology
  • Mitochondrial function

Background:

  • Cell viability assessment is crucial in biological and medical research.
  • There is a need for rapid, reliable, and wash-free fluorescent probes for complex cell viability evaluations.
  • The electron transport chain (ETC) is an early indicator of cellular distress, offering potential for viability assessment.

Purpose of the Study:

  • To introduce Rhodalive, a novel mitochondria-targeted, ETC-activated fluorescence turn-on probe.
  • To enable wash-free, spatiotemporal, and super-resolution fluorescence imaging of active mitochondria for single-cell viability assessment.
  • To demonstrate Rhodalive's utility in distinguishing live from fixed cells and quantifying cellular damage.

Main Methods:

  • Development of Rhodalive, a mitochondria-targeted probe activated by ETC-released electrons.
  • Utilizing Rhodalive for wash-free, super-resolution fluorescence imaging of mitochondria.
  • Applying Rhodalive to assess H2O2-induced cell damage and photodamage from blue light exposure and photodynamic therapy.

Main Results:

  • Rhodalive is activated by free electrons from the ETC and localizes to mitochondria.
  • The probe enables wash-free, spatiotemporal, and super-resolution imaging of active mitochondria.
  • Rhodalive successfully distinguishes live from fixed cells, quantifies H2O2-induced damage, and visualizes photodamage.

Conclusions:

  • Rhodalive is a convenient and reliable tool for dynamic single-cell viability assessment.
  • The probe is promising for evaluating early mitochondrial dysfunction.
  • Rhodalive has potential applications in drug discovery and advancing cell viability research.