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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Although all next-generation methods use different technologies, they all share a set of standard features....

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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Related Experiment Video

Updated: May 29, 2025

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Progress in Aptamer Research and Future Applications.

Song Liu¹, Xiaolu Li¹, Huyang Gao²

¹Beijing Anzhen Hospital, Capital Medical University, Experimental Research Center, Beijing Institute of Heart Lung and Blood Vessel Disease, Beijing, China.

|February 4, 2025

Summary

This summary is machine-generated.

Aptamers, short nucleic acid sequences, offer a promising alternative to antibodies for diagnostics and therapeutics. This review highlights advances in aptamer generation, screening, and clinical applications, while also addressing current challenges.

Keywords:

Antibody Aptamer Aptamer characterization Small molecule Systematic evolution of ligands by exponential enrichment (SELEX)

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Area of Science:

Biotechnology and Molecular Biology
Nucleic Acid Therapeutics
Diagnostic Development

Background:

Aptamers are single-stranded DNA, RNA, or synthetic XNA molecules with high specificity and affinity for target molecules.
They serve as viable alternatives to antibodies in various biological applications.
Aptamers demonstrate significant potential in diagnostics, targeted drug delivery, and disease treatment.

Purpose of the Study:

To review recent advancements in aptamer technology.
To emphasize aptamer generation, screening, and diverse applications.
To discuss challenges and future directions in aptamer research and clinical use.

Main Methods:

The review focuses on the systematic evolution of exponentially enriched ligands (SELEX) process for aptamer identification.
It examines various stages of aptamer generation and screening.
Literature review of current research on aptamer applications and clinical translation.

Main Results:

Aptamers are increasingly utilized in basic research and clinical diagnostics.
Significant progress has been made in developing aptamers for therapeutic applications.
The review covers advances in small molecule aptamers and their development.

Conclusions:

Aptamers represent a rapidly advancing field with broad potential in medicine and research.
Further research is needed to overcome existing challenges for wider clinical adoption.
Continued innovation in aptamer generation and application development is crucial.