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Regulation of monocyte function by complement activation products.

R M Antrum, J S Solomkin

    The Journal of Surgical Research
    |May 1, 1985
    PubMed
    Summary
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    Human monocytes exposed to zymosan-activated serum (ZAS) show reduced chemotaxis to C5a but not FMLP. Monocytes actively absorb C5a, indicating specific regulation by C5a and its metabolite C5adesArg.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Monocytes play a crucial role in immune responses.
    • Chemotaxis is essential for monocyte migration to inflammatory sites.
    • Complement components like C5a and formylated peptides (e.g., FMLP) are potent chemoattractants.

    Purpose of the Study:

    • To investigate the specific chemotactic responses of normal human monocytes to C5a and FMLP.
    • To determine the effect of pre-exposure to ZAS and FMLP on monocyte chemotaxis.
    • To explore the interaction between monocytes and C5a.

    Main Methods:

    • Isolation of normal human monocytes using Percoll density gradient centrifugation.
    • Chemotaxis assays measuring monocyte migration towards C5a and FMLP.
    • Exposure of monocytes to zymosan-activated serum (ZAS) and varying concentrations of FMLP.

    Related Experiment Videos

  • Measurement of C5a absorption by monocytes from ZAS.

    • Main Results:

    • Monocytes pre-exposed to low concentrations of ZAS exhibited chemotactic deactivation to C5a, but retained responsiveness to FMLP.
    • C5a was significantly more potent (40x) than C5adesArg in reducing chemotaxis to activated serum.
    • Exposure to FMLP reduced monocyte chemotaxis to FMLP but did not affect chemotaxis to activated serum.
    • Monocytes actively absorbed C5a from C5a-rich ZAS solutions.

    Conclusions:

    • Monocyte chemotaxis is specifically regulated by C5a and its metabolite C5adesArg.
    • Monocytes possess mechanisms to modulate their response to chemoattractants like C5a.
    • These findings contribute to understanding monocyte function in immune regulation.