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Related Experiment Video

Updated: May 29, 2025

An Assay for Quantifying Protein-RNA Binding in Bacteria

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

RBP-Tar - a searchable database for experimental RBP binding sites.

Katarina Gresova^1,2, Tomas Racek^1,2, Vlastimil Martinek^1,2

¹National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic.

|February 6, 2025

Summary

This summary is machine-generated.

We developed RBP-Tar, a web server and database that provides access to RNA-binding protein (RBP) binding site data from eCLIP experiments. This resource helps researchers investigate RBP functions in gene regulation and disease.

Keywords:

CLIP RBP RNA Binding Proteins Web-server

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Last Updated: May 29, 2025

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PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

Published on: July 2, 2010

Area of Science:

Molecular Biology
Genomics
Bioinformatics

Background:

RNA-binding proteins (RBPs) are crucial regulators of gene expression.
Identifying RBP binding sites is essential for understanding cellular functions, development, and disease.
Enhanced CLIP (eCLIP) is a key experimental method for mapping RBP binding sites.

Purpose of the Study:

To create a user-friendly web server and database for accessing RBP binding site data.
To enhance scientific accessibility of eCLIP data for 167 human RBPs.
To facilitate research on RBP-mediated gene regulation.

Main Methods:

Development of the RBP-Tar web server and database.
Utilization of eCLIP data for 167 RBPs mapped to the human genome.
Implementation of search functionalities by genomic location and RBP name.

Main Results:

RBP-Tar provides easy access to experimentally validated RBP binding sites.
Researchers can retrieve binding site information for specific genes or RBPs.
The database integrates eCLIP data for a significant number of RBPs.

Conclusions:

RBP-Tar serves as a valuable resource for the scientific community.
The platform facilitates the study of RBP functions in various biological contexts.
Future updates will include additional CLIP experiment datasets.