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Updated: Jul 1, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
Published on: February 28, 2015
A system for in vitro selection of fully 2'-modified RNA aptamers
Emily D Ziperman1, Kate B Fitzpatrick1, Malavika A Nair1
1Department of Chemistry, Brandeis University, Waltham, Massachusetts, USA. kraussi@brandeis.edu.
None:
SFM4-3, KOD DGLNK, and Therminator polymerase are investigated for their compatibility with SELection with Modified Aptamers (SELMA), an aptamer discovery method that enables incorporation of large nucleobase modifications such as glycans. We demonstrated that with suitable modifications to the primer design and protocol, these enzymes are compatible with SELMA, enabling 2'-fluoro or 2'-methoxy ribose modifications at all positions. In the case of 2'-fluoro modifications, Therminator exhibits cleaner incorporation of an alkyne-modified nucleobase for click chemistry.
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