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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Reference Intervals and Comparative Analysis of Reticulocyte Counts Using the Mindray BC-6200, Flow Cytometry, and

Hathaiphon Aphaiwiwat¹, Chutitorn Ketloy^2,3, Tapakorn Thepnarin⁴

¹Program in Medical Sciences, Division of Clinical Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

International Journal of Laboratory Hematology

|February 7, 2025

Summary

The Mindray BC-6200 analyzer offers reliable reticulocyte counting, showing excellent precision, low carryover, and time stability. It serves as a strong alternative to manual microscopy and flow cytometry for assessing erythropoiesis.

Keywords:

automated hematology analyzer flow cytometry method manual microscopic method reference intervals reticulocyte count

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Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

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Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes

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Published on: March 22, 2022

Area of Science:

Hematology
Clinical Chemistry
Laboratory Medicine

Background:

Reticulocyte counting is crucial for evaluating erythropoiesis.
Traditional methods include manual microscopy (MM) and flow cytometry (FC).
Analytical performance of automated analyzers needs thorough evaluation.

Purpose of the Study:

To evaluate the analytical performance of the Mindray BC-6200 for reticulocyte counting.
To compare its accuracy against manual microscopy and flow cytometry.
To establish reference intervals for the Thai population.

Main Methods:

Analytical performance assessed for imprecision, carryover, and time stability.
Sixty healthy and 182 pathological Thai samples were analyzed.
Results compared with manual microscopy and flow cytometry.

Main Results:

Imprecision (%CV) was below manufacturer claims.
Carryover was <0.001% and time stability was excellent up to 24 hours.
Strong correlation (r>0.9785) observed between automated, MM, and FC methods.

Conclusions:

Mindray BC-6200 is a reliable alternative for reticulocyte counting.
The analyzer demonstrates good correlation, precision, and stability.
Further studies are needed to assess clinical utility in hematological diagnostics.