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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: May 28, 2025

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

Imaging flow cytometry with a real-time throughput beyond 1,000,000 events per second.

Jiehua Zhou¹, Liye Mei^1,2, Mingjie Yu¹

¹The Institute of Technological Sciences, Wuhan University, Wuhan, 430072, China.

Light, Science & Applications

|February 9, 2025

Summary

This summary is machine-generated.

This study introduces a new imaging flow cytometry (IFC) system achieving over 1,000,000 events per second, significantly advancing high-throughput cell analysis for biomedical applications.

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Area of Science:

Biomedical Engineering
Cell Biology
Analytical Chemistry

Background:

Imaging flow cytometry (IFC) merges microscopy and flow cytometry for cell analysis.
Current IFC systems are limited to 10,000 events per second (eps), hindering large-scale studies.
High-throughput cell analysis is crucial for biomedicine, green energy, and environmental monitoring.

Purpose of the Study:

To develop an IFC system with real-time throughput exceeding 1,000,000 eps.
To overcome the throughput limitations of existing IFC technologies.
To enable highly efficient and accurate cell measurement.

Main Methods:

Integration of optical time-stretch (OTS) imaging.
Utilization of microfluidic-based cell manipulation.
Implementation of online image processing for real-time analysis.

Main Results:

Achieved real-time throughput greater than 1,000,000 eps.
Successfully imaged cells at speeds up to 15 m/s with 780 nm resolution.
Validated system performance by identifying malignancies in colorectal samples.

Conclusions:

This novel IFC system sets a new record for throughput in the field.
The technology has the potential to revolutionize cell analysis with enhanced efficiency and accuracy.
Enables intelligent measurement for diverse scientific and medical applications.