Related Experiment Video
Updated: May 28, 2025
07:19
Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
Published on: June 28, 2017
10.2K
Imaging flow cytometry with a real-time throughput beyond 1,000,000 events per second
Jiehua Zhou1, Liye Mei1,2, Mingjie Yu1
1The Institute of Technological Sciences, Wuhan University, Wuhan, 430072, China.
Light, Science & Applications
|February 9, 2025
Summary
This study introduces a new imaging flow cytometry (IFC) system achieving over 1,000,000 events per second, significantly advancing high-throughput cell analysis for biomedical applications.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Analytical Chemistry
Background:
- Imaging flow cytometry (IFC) merges microscopy and flow cytometry for cell analysis.
- Current IFC systems are limited to 10,000 events per second (eps), hindering large-scale studies.
- High-throughput cell analysis is crucial for biomedicine, green energy, and environmental monitoring.
Purpose of the Study:
- To develop an IFC system with real-time throughput exceeding 1,000,000 eps.
- To overcome the throughput limitations of existing IFC technologies.
- To enable highly efficient and accurate cell measurement.
Main Methods:
- Integration of optical time-stretch (OTS) imaging.
- Utilization of microfluidic-based cell manipulation.
- Implementation of online image processing for real-time analysis.
Main Results:
- Achieved real-time throughput greater than 1,000,000 eps.
- Successfully imaged cells at speeds up to 15 m/s with 780 nm resolution.
- Validated system performance by identifying malignancies in colorectal samples.
Conclusions:
- This novel IFC system sets a new record for throughput in the field.
- The technology has the potential to revolutionize cell analysis with enhanced efficiency and accuracy.
- Enables intelligent measurement for diverse scientific and medical applications.
