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Characterisation and analysis of mRNA critical quality attributes using liquid chromatography based methods.

Alexandra L J Webb1, Emma N Welbourne1, Caroline A Evans1

  • 1School of Chemical, Materials and Biological Engineering, University of Sheffield, Sheffield, S1 3JD, UK.

Journal of Chromatography. A
|February 13, 2025
PubMed
Summary

Messenger RNA (mRNA) analysis is crucial for developing new therapies beyond vaccines. This review details high-performance liquid chromatography methods for characterizing mRNA critical quality attributes.

Keywords:
Critical quality attributesHPLCMass spectrometrymRNAmRNA vaccines/therapeutics

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