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Updated: Jul 15, 2026

Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue
10:12

Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue

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Patterns of Increased Cardiomyocyte Ploidy in Myocardial Hypertrophy of Various Origins

T V Sukhacheva1,2, R A Serov3, A I Kim3

  • 1A. N. Bakulev Center for Cardiovascular Surgery, Ministry of Health of the Russian Federation, Moscow, Russia. sukhachevat@gmail.com.

Bulletin of Experimental Biology and Medicine
|February 13, 2025
PubMed

Insights

Cardiomyocyte (CMC) polyploidy drives hypertrophic growth in heart conditions like hypertrophic cardiomyopathy (HCM) and tetralogy of Fallot (TF). HCM hypertrophy stems from polyploidization, while TF involves increased multinucleated CMCs.

Area of Science:

  • Cardiovascular Biology
  • Cell Biology
  • Pathology

Background:

  • Cardiomyocyte (CMC) ploidy is linked to hypertrophic growth potential.
  • Understanding CMC ploidy and size changes is crucial in pediatric and adult cardiovascular diseases.

Purpose of the Study:

  • To investigate alterations in CMC ploidy and interventricular septum size in hypertrophic cardiomyopathy (HCM) and tetralogy of Fallot (TF).
  • To compare these changes between patient groups and healthy controls, and across different age groups.

Main Methods:

  • Analysis of cardiomyocyte ploidy and cell size in patient samples.
  • Comparison of cellular metrics between patients with HCM, TF, and control individuals.
  • Assessment of the proportion of mononucleated and multinucleated cardiomyocytes.

Main Results:

  • CMC ploidy was 1.5-2 fold higher in HCM and TF patients compared to controls, irrespective of age.
  • CMC size increased by 1.3-2 times in HCM and TF patients, with a more significant increase in HCM.
  • Multinucleated CMCs were less frequent in HCM but nearly doubled in TF patients.

Conclusions:

  • Myocardial hypertrophy in HCM is primarily driven by polyploidy in mononucleated CMCs.
  • In tetralogy of Fallot, increased myocardial hypertrophy is associated with a higher proportion of multinucleated CMCs.

Keywords:
cardiomyocyte ploidyhypertrophic cardiomyopathyhypertrophymultinucleated cardiomyocytestetralogy of Fallot

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