Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Emission Spectroscopy: Overview01:20

Atomic Emission Spectroscopy: Overview

1.4K
Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
1.4K
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

141
AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
141
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.3K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.3K
Imaging Studies II: Positron Emission Tomography and Scintigraphy01:25

Imaging Studies II: Positron Emission Tomography and Scintigraphy

68
Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET
68
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

180
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
180
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.1K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Based on artificial intelligence-assisted generation and in-depth in-silico evaluation of potential inhibitors targeting Stearoyl-CoA desaturase 1 (SCD-1).

Scientific reports·2026
Same author

Mechanistic Elucidation of Liujun Jiaoxian Tang in Management of Sepsis Through Metabolomics and Network Pharmacology.

Biomedical engineering and computational biology·2026
Same author

Deciphering the Transcriptomic Dynamics of Self-Incompatibility in Yellow Passion Fruit: Evidence of Modified Sporophytic Mechanism.

Plants (Basel, Switzerland)·2026
Same author

Comparative analysis of short-term outcomes, inflammatory markers, and complications in robotic-assisted <i>vs.</i> traditional thoracoscopic surgery for complex pulmonary segmentectomy.

Journal of thoracic disease·2026
Same author

Meta-transcriptomic characterization reveals viral species with zoonotic potential in Rhipicephalus microplus and Haemaphysalis bispinosa ticks in Pakistan.

Veterinary research·2026
Same author

An emerging tick-borne spotted fever group Rickettsia in China: Isolation, genomics, and human infection of Rickettsia koreansis.

The Journal of infection·2026
Same journal

BPENet: Boundary perception enhancement network for retinal vessel and coronary angiogram segmentation.

Journal of X-ray science and technology·2026
Same journal

Semi-supervised YOLO-DEP for high-resolution X-ray component localization and counting.

Journal of X-ray science and technology·2026
Same journal

Attention based multi-scale edge-aware segmentation and convolutional transformer framework for automated glaucoma detection from fundus images.

Journal of X-ray science and technology·2026
Same journal

Improving the robustness of radiomic features to patient size variations in CBCT imaging for radiotherapy.

Journal of X-ray science and technology·2026
Same journal

DH-OOD: A decoupled hybrid framework for robust skin lesion classification via semantic-structural fusion.

Journal of X-ray science and technology·2026
Same journal

Development and evaluation of deep learning models for automatic coronary stenosis segmentation in X-ray angiography.

Journal of X-ray science and technology·2026
See all related articles

Related Experiment Video

Updated: May 27, 2025

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles
10:00

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles

Published on: July 5, 2016

11.7K

Quantitative elemental sensitive imaging based on K-edge subtraction tomography.

Yichi Zhang1,2,3, Fen Tao1,2,3, Ruoyang Gao1,2,3

  • 1Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.

Journal of X-Ray Science and Technology
|February 20, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a new quantitative method for elemental analysis using K-edge subtraction (KES) tomography. The technique achieves nanometer-scale elemental imaging with high accuracy, enabling precise elemental composition assessments.

Keywords:
full-field nano-CTgrayscalek-edge subtraction tomographyquantitative elemental sensitive imaging

More Related Videos

Atom Probe Tomography Analysis of Exsolved Mineral Phases
08:14

Atom Probe Tomography Analysis of Exsolved Mineral Phases

Published on: October 25, 2019

7.1K
Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
09:47

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

4.7K

Related Experiment Videos

Last Updated: May 27, 2025

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles
10:00

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles

Published on: July 5, 2016

11.7K
Atom Probe Tomography Analysis of Exsolved Mineral Phases
08:14

Atom Probe Tomography Analysis of Exsolved Mineral Phases

Published on: October 25, 2019

7.1K
Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
09:47

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

4.7K

Area of Science:

  • Materials Science
  • Physics
  • Medical Imaging

Background:

  • K-edge subtraction (KES) tomography is a powerful technique for elemental sensitive imaging.
  • Existing KES tomography methods primarily offer qualitative elemental analysis.
  • There is a need for quantitative elemental assessment in KES tomography.

Purpose of the Study:

  • To develop a novel method for quantitative elemental analysis using KES tomography.
  • To enable precise and convenient elemental composition measurements.
  • To expand the applications of KES tomography.

Main Methods:

  • Established a linear correlation between slice grayscale and linear absorption coefficients.
  • Utilized grayscale data for quantitative estimation of elemental compositions.
  • Validated the method using standard and actual samples.

Main Results:

  • Achieved nanometer-resolved quantitative elemental sensitive imaging.
  • Demonstrated a relative error of less than 3% in target elemental content.
  • Confirmed the accuracy and validity of the proposed quantitative method.

Conclusions:

  • The developed method provides a novel approach for precise quantitative elemental analysis.
  • This technique is expected to broaden the applications of KES tomography.
  • Offers a more convenient way to perform quantitative elemental assessments.