Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Screening effects of China's driver's license test reform: evidence from regression discontinuity design.

Accident; analysis and prevention·2026
Same author

Vertically Stacked Indium Gallium Zinc Oxide-Based Three-Dimensional Integrated Circuits.

ACS nano·2026
Same author

Toward autonomous robotic-assisted and microrobotic surgery.

Science advances·2026
Same author

Adaptive Neural Reorganization Enables Real-Time Finger-Level Robotic Control in BCI-Naïve Stroke Survivors.

bioRxiv : the preprint server for biology·2026
Same author

Discovery of Dihydropyrimido-Pyrimidine-Based Bone Marrow Tyrosine Kinase Gene in Chromosome X Protein Proteolysis-Targeting Chimeras for the Treatment of Prostate Cancer.

ACS medicinal chemistry letters·2026
Same author

Synergistic Electronic and Steric Engineering of Thiophene Donors in Covalent Organic Frameworks for Efficient CO<sub>2</sub> Photoconversion.

Angewandte Chemie (International ed. in English)·2026

Related Experiment Video

Updated: May 27, 2025

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
10:37

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Published on: March 14, 2021

6.3K

3D Spatiotemporal Activation Mapping of Cardiac Organoids Using Conformal Shell Microelectrode Arrays (MEAs).

Soo Jin Choi1, Zhaoyu Liu2, Feiyu Yang2

  • 1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218.

Research Square
|February 20, 2025
PubMed
Summary
This summary is machine-generated.

New shell microelectrode arrays (MEAs) map cardiac organoid electrical activity in 3D. This technology enables detailed analysis of signal propagation and cardiotoxicity screening for improved cardiac disease modeling.

More Related Videos

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices
09:35

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices

Published on: June 16, 2020

9.7K
Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform
10:42

Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform

Published on: June 15, 2021

4.9K

Related Experiment Videos

Last Updated: May 27, 2025

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
10:37

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Published on: March 14, 2021

6.3K
Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices
09:35

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices

Published on: June 16, 2020

9.7K
Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform
10:42

Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform

Published on: June 15, 2021

4.9K

Area of Science:

  • Biomedical Engineering
  • Cardiovascular Research
  • Organoid Technology

Background:

  • Cardiac organoids are valuable models for studying heart development and diseases.
  • Traditional 2D microelectrode arrays (MEAs) lack the ability to capture 3D electrical signal propagation in cardiac organoids.

Purpose of the Study:

  • To develop and validate a novel, shape-adaptive shell MEA for comprehensive electrophysiological mapping of cardiac organoids.
  • To enable 3D spatiotemporal analysis of electrical activity and facilitate cardiotoxicity screening.

Main Methods:

  • Fabrication of on-chip, programmable, shape-adaptive shell MEAs with tunable dimensions and electrode layouts.
  • Encapsulation of spherical cardiac organoids for precise surface electrical mapping.
  • Simultaneous electrophysiological and calcium imaging for validation of signal propagation.

Main Results:

  • Generation of 3D isochrone maps and conduction velocity vectors for cardiac organoids.
  • Demonstration of accurate electrophysiological signal propagation mapping across the organoid surface.
  • Successful monitoring of electrophysiological changes in response to cardiotoxic drugs over nine days.

Conclusions:

  • Shell MEAs provide unprecedented 3D electrophysiological mapping capabilities for cardiac organoids.
  • This technology significantly advances cardiac organoid development, disease modeling, and drug screening platforms.
  • Shell MEAs combined with spatiotemporal mapping are poised to accelerate cardiovascular research and therapeutic development.