Related Experiment Video
Updated: May 26, 2025
10:12
Visualization of G3BP Stress Granules Dynamics in Live Primary Cells
Published on: May 21, 2014
18.3K
The G3BP1 RNP complex at focal adhesion tunes cell migration
Zhiying Yao1, Ziqiu Wang1, Peiguo Yang1
1School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China.
Trends in Cell Biology
|February 21, 2025
Summary
Stress granules (SGs) are mesoscale assemblies of ribonucleoprotein (RNP) complexes. A new study reveals G3BP1 forms focal adhesion-localized RNP complexes, impacting cell migration.
Area of Science:
- Cell biology
- Molecular biology
- Biochemistry
Background:
- Ribonucleoprotein (RNP) complexes form various mesoscale assemblies, such as stress granules (SGs).
- The precise functions and regulatory mechanisms of soluble RNP complexes remain incompletely understood.
- G3BP1 is recognized as a crucial component in the assembly of stress granules.
Purpose of the Study:
- To investigate the role of G3BP1 beyond stress granule formation.
- To explore the localization and function of RNP complexes involving G3BP1 at focal adhesions.
- To understand how G3BP1-mediated RNP complexes influence cellular processes like migration.
Main Methods:
- Immunofluorescence microscopy to visualize protein localization.
- Biochemical assays to study RNP complex formation.
- Cell migration assays to assess functional impact.
Main Results:
- G3BP1 localizes to focal adhesions, forming distinct RNP complexes at these sites.
- These focal adhesion-localized RNP complexes are functionally distinct from stress granules.
- Disruption of these complexes affects cell migration dynamics.
Conclusions:
- G3BP1 plays a dual role in RNP complex formation, contributing to both stress granules and focal adhesion-localized complexes.
- Focal adhesion-localized RNP complexes mediated by G3BP1 are regulators of cell migration.
- This finding expands the known functions of G3BP1 and RNP complexes in cellular regulation.
Related Concept Videos
01:17Intracellular Signaling Affects Focal Adhesions
2.5K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.5K
01:32Cytoskeletal Coordination in Cell Migration
4.7K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.7K
01:09Cell Migration
16.9K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
16.9K
01:21Cell Polarization by Rho Proteins
2.6K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.6K
01:24Small GTPases - Ras and Rho
3.9K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
3.9K
01:18Role of Myosin in Cell Migration
2.2K
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
2.2K