Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Responses to Drought and Flooding02:41

Responses to Drought and Flooding

Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
Excitatory and Inhibitory Effects of Neurotransmitters01:29

Excitatory and Inhibitory Effects of Neurotransmitters

When an action potential reaches the presynaptic axon terminal, it releases neurotransmitters from the neuron into the synaptic cleft at a chemical synapse. The released neurotransmitter can be excitatory or inhibitory. The critical criteria commonly used to determine whether a molecule is a neurotransmitter at a chemical synapse are the molecule's presence in the presynaptic neuron. Second, its release is in response to strong presynaptic depolarization. And lastly, the presence of specific...
Drugs Affecting Neurotransmitter Release or Uptake01:21

Drugs Affecting Neurotransmitter Release or Uptake

Certain drugs can affect how neurotransmitters called catecholamines, are released or taken back up in the adrenergic neuron. They can have different effects on the body's sympathetic transmission. Reserpine, a natural compound found in the Rauwolfia shrub, blocks a transporter called vesicular monoamine transporter (VMAT), which leads to a buildup of catecholamines in the cell and reduces sympathetic transmission. Another drug called guanethidine works in multiple ways, including blocking...
Disorders of the Nervous Tissue01:28

Disorders of the Nervous Tissue

Nervous tissue is a vital component of the human body's communication system, enabling us to perceive and respond to stimuli. However, like all other tissues, it is vulnerable to disorders and diseases that can significantly impact our neurological functioning.
Homeostatic Imbalances:
Alzheimer's disease manifests as a gradual decline in memory and cognitive abilities, attributed to the buildup of amyloid plaques and neurofibrillary tangles in the brain.
Parkinson's disease arises from the...
Drugs Affecting Neurotransmitter Synthesis01:29

Drugs Affecting Neurotransmitter Synthesis

Drugs affecting neurotransmitter synthesis can impact the adrenergic neuron and the synthesis of neurotransmitters. For example, α-methyltyrosine and carbidopa target specific enzymes involved in catecholamine synthesis. α-methyltyrosine inhibits the enzyme tyrosine hydroxylase, which converts tyrosine into dopamine. By blocking this enzyme, α-methyltyrosine reduces dopamine production and other catecholamines. Carbidopa, on the other hand, inhibits the enzyme dopa decarboxylase, which converts...
Dementia l: Introduction01:22

Dementia l: Introduction

Dementia is an acquired, progressive syndrome characterized by a decline in multiple cognitive domains severe enough to impair daily functioning and reduce independence. Although memory loss is a central feature, the diagnosis requires additional deficits involving language, executive function, visuospatial skills, judgment, calculation, or abstract reasoning. These cognitive impairments reflect underlying neurodegenerative or vascular processes that gradually disrupt neuronal networks...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical, in vitro, and in vivo evidence of WAPL as a cohesinopathy-associated gene and phenotypic driver of 10q22.3q23.2 genomic disorder.

American journal of human genetics·2026
Same author

Cardiomyopathy and mitochondrial encephalomyopathy in a female child associated with a heterozygous X-linked AIFM1 variant.

Molecular and cellular pediatrics·2026
Same author

Telomere biology disorders associated with childhood interstitial lung disease.

Clinical and experimental pediatrics·2026
Same author

Evidence supporting the role of GIGYF2 in synapse development and autism.

Molecular psychiatry·2026
Same author

In vitro study of TSC1 deficiency in preadipocytes: insights into development and treatment options for tuberous sclerosis related lipomatosis.

Orphanet journal of rare diseases·2026
Same author

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway.

FEBS open bio·2026

Related Experiment Video

Updated: Jun 20, 2026

Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations
07:18

Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations

Published on: September 30, 2010

Drosophila WDFY3/Bchs overexpression impairs neural function.

Marek B Körner1,2, Akhil Velluva1, Linnaeus Bundalian1

  • 1Institute of Human Genetics, University of Leipzig Medical Center, Leipzig, Germany.

Journal of Neurogenetics
|February 25, 2025
PubMed
Summary

Upregulating the WDFY3 gene in glial or neuronal cells impairs autophagy and locomotion. This study reveals detrimental effects of WDFY3 gene upregulation in the central nervous system.

Keywords:
BchsWDFY3autophagyneurodevelopmental delaytranscriptomics

More Related Videos

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
10:13

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

Published on: November 7, 2017

In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster
10:00

In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

Published on: July 11, 2019

Related Experiment Videos

Last Updated: Jun 20, 2026

Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations
07:18

Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations

Published on: September 30, 2010

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
10:13

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

Published on: November 7, 2017

In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster
10:00

In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster

Published on: July 11, 2019

Area of Science:

  • Neuroscience
  • Genetics
  • Cell Biology

Background:

  • Pathogenic variants in WDFY3 are linked to neurodevelopmental disorders.
  • WDFY3 encodes the autophagy adaptor protein ALFY.
  • The effects of WDFY3 upregulation in the central nervous system (CNS) are largely unknown.

Purpose of the Study:

  • To investigate the consequences of WDFY3 ortholog (Bchs) upregulation in Drosophila glial and neuronal cells.
  • To understand the impact of Bchs overexpression on autophagy, locomotion, and overall morphology.
  • To identify genes and pathways affected by Bchs upregulation in different cell types.

Main Methods:

  • Overexpression of the Drosophila WDFY3 ortholog, Bchs, in glial and neuronal cells.
  • Assessment of autophagy and locomotion.
  • Analysis of ventral nerve cord (VNC) size and glial nuclei number.
  • Gene expression profiling to identify differentially expressed genes.
  • Gene Ontology (GO) analysis of affected genes.

Main Results:

  • Glial or neuronal Bchs overexpression impaired autophagy and locomotion.
  • Glial Bchs overexpression increased VNC size and glial nuclei number.
  • Neuronal Bchs overexpression affected wing and thorax morphology.
  • 79 differentially expressed genes overlapped between glial and neuronal Bchs overexpression.
  • Neuronal Bchs overexpression led to differential gene expression in autophagy and mitochondrial function categories.

Conclusions:

  • WDFY3 (Bchs) upregulation in either glial or neuronal cells has detrimental effects on neural function.
  • Both cell types show impaired autophagy and locomotion upon Bchs upregulation.
  • Glial and neuronal Bchs upregulation impact distinct developmental and cellular processes.
  • Gene expression analysis reveals conserved and cell-specific responses to WDFY3 upregulation.