Antimicrobial Denture Material Synthesized from Poly(methyl methacrylate) Enriched with Cannabidiol Isolates

Area of Science:

• Biomaterials Science
• Oral Microbiology
• Nanotechnology

Background:

• Denture-associated infections are common, necessitating antimicrobial enhancements for poly(methyl methacrylate) (PMMA) dentures.
• Cannabidiol (CBD) exhibits antimicrobial properties effective against oral bacteria.

Purpose of the Study:

• To develop and evaluate CBD-nanoparticle-infused PMMA denture coatings for antimicrobial efficacy.
• To assess the impact of CBD coatings on dental biofilm formation.

Main Methods:

• CBD nanoparticles were synthesized and incorporated into PMMA coatings using UV curing.
• Characterization involved ¹H NMR, SEM, and FTIR spectroscopies.
• Antimicrobial activity and biofilm reduction were tested against *Staphylococcus aureus*, *Escherichia coli*, and *Streptococcus agalactiae*.

Main Results:

• CBD coatings showed significant bactericidal effects against Gram-positive bacteria (MIC: 2-2.5 µg/mL, MBC: 10-20 µg/mL).
• CBD was ineffective against planktonic Gram-negative bacteria but reduced overall biofilm growth by 99% on PMMA.
• CBD disrupted bacterial cell walls, leading to lysis and demonstrating effective molecule release.

Conclusions:

• CBD-infused PMMA denture coatings present a promising antibiotic-free strategy to combat oral biofilms and plaque.
• This approach can enhance denture hygiene and improve patient oral health outcomes.