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Updated: May 25, 2025

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice
Published on: November 27, 2019
INTERMITTENT FASTING (5:2) VS. NON-FASTING: A COMPARATIVE ANALYSIS OF ANTHROPOMETRIC PARAMETERS, DEPRESSION, AND
R Othman1, I Karmous2, R Mizouri3
11University of Tunis el manar faculty of medicine of Tunis; 2Institut National de Nutrition et de Technologie Alimentaire De Tunis.
The 5:2 intermittent fasting diet (IF 5:2) impacts nutritional intake, showing lower protein, lipid, and carbohydrate levels in fasters. This dietary approach was also linked to fewer depressive symptoms compared to non-fasting individuals.
Area of Science:
- Nutrition Science
- Dietary Interventions
- Metabolic Health
Background:
- Intermittent fasting, specifically the 5:2 diet, is a popular strategy for weight management and improving metabolic health.
- Understanding the nutritional and psychological effects of the 5:2 diet is crucial for public health guidance.
Purpose of the Study:
- To compare anthropometric and nutritional profiles between individuals following the 5:2 intermittent fasting diet (IF 5:2) and non-fasting individuals.
- To assess differences in depressive symptoms and stress levels between the two groups.
Main Methods:
- A cross-sectional study involving 71 participants (35 IF 5:2, 36 non-fasting), matched for age and gender.
- Data collection included questionnaires, anthropometric measurements, dietary surveys (24-hour recall, dietary habits), and validated scales for anxiety, depression (Hospital Anxiety and Depression scale), and stress (Perceived Stress Scale).
Main Results:
- No significant differences in weight or BMI were observed between the groups.
- Fasting individuals consumed lower levels of proteins, lipids, and carbohydrates, while non-fasting individuals had higher intake of potassium, phosphorus, and sugars.
- The IF 5:2 group reported fewer depressive symptoms (p=0.04), but stress levels were comparable (p=0.19).
Conclusions:
- The 5:2 intermittent fasting diet significantly alters nutritional intake compared to habitual diets.
- The IF 5:2 diet may be associated with reduced depressive symptoms, warranting further investigation.
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