Multi-spectral laser speckle contrast imaging for depth-resolved blood perfusion assessment
Liban Hussein1, Sajjad Moazeni1
1University of Washington, Department of Electrical and Computer Engineering, Seattle, Washington, United States.
Journal of Biomedical Optics
|February 26, 2025
Summary
Multi-spectral laser speckle contrast imaging (MS-LSCI) enhances depth-resolved blood perfusion assessment. This technique uses multiple wavelengths and correlation methods for improved accuracy in biomedical applications like burn diagnostics.
Area of Science:
- Biomedical Optics
- Medical Imaging
Background:
- Laser speckle contrast imaging (LSCI) is vital for assessing blood perfusion in tissues.
- Conventional LSCI offers 2D imaging but lacks depth resolution, limiting its use in deeper tissue analysis.
- Improved depth profiling in LSCI can advance applications like vascular imaging and burn diagnostics.
Purpose of the Study:
- To investigate multi-spectral laser speckle contrast imaging (MS-LSCI) for depth-resolved blood perfusion assessment.
- To utilize multiple laser wavelengths and advanced correlation techniques for enhanced depth localization.
Main Methods:
- Fabricated tissue phantoms to simulate blood vessels at varying depths.
- Employed a range of laser wavelengths (blue to near-infrared) for experiments.
- Utilized the visibility parameter () for depth correlation and spectral wavelength mapping to improve signal quality.
Main Results:
- MS-LSCI achieved improved depth profiling accuracy across different laser wavelengths.
- Spectral wavelength mapping enhanced signal quality, especially for wavelengths with limited tissue penetration.
- The visibility parameter consistently correlated depth across phantom models, validated by human hand imaging.
Conclusions:
- MS-LSCI shows significant potential for non-invasive, depth-resolved blood perfusion imaging.
- The multi-wavelength approach enhances LSCI's utility for biomedical applications, including burn wound assessment and vascular imaging.
