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Related Concept Videos

Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

176
Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
176

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Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry
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Saliva analysis using metal-organic framework-coated miniaturized vials.

Iván Taima-Mancera1, María J Trujillo-Rodríguez1, Jorge Pasán2

  • 1Laboratorio de Materiales para Análisis Químico (MAT4LL), Departamento de Química, Unidad Departamental de Química Analítica, Universidad de La Laguna (ULL), 38206, San Cristóbal de La Laguna, Spain; Unidad de Investigación de Bioanalítica y Medio Ambiente, Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias (IUETSPC), ULL, 38206, San Cristóbal de La Laguna, Spain.

Analytica Chimica Acta
|February 27, 2025
PubMed
Summary

A novel metal-organic framework (MOF)-coated vial enables simple and rapid analysis of saliva using thin-film solid-phase microextraction. This reusable device overcomes protein clogging issues, offering sensitive detection of bisphenols with minimal solvent use.

Keywords:
CoatingsIn vial microextractionLiquid chromatographyMetal-organic frameworksSalivaSample preparationThin film solid-phase microextraction

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Bioanalysis

Background:

  • In-vial microextraction offers simplicity and speed but has limited applications, especially for complex biological samples.
  • Metal-organic frameworks (MOFs) show promise as adsorbents, but their use in bioanalysis, particularly with high-protein samples like saliva, is underexplored.
  • Developing stable, reusable sorbents for low-volume bioanalytical samples is crucial.

Purpose of the Study:

  • To develop and evaluate a novel metal-organic framework (MOF)-coated vial for in-vial microextraction of saliva.
  • To assess the applicability of this technique for analyzing bisphenols in complex biological matrices.
  • To address challenges of protein adsorption and ensure reusability of the analytical device.

Main Methods:

  • A 2 mL vial was uniformly coated with a MOF material for thin-film solid-phase microextraction.
  • A simple cleaning protocol was implemented to mitigate protein clogging issues.
  • Seven bisphenols were analyzed in saliva samples using liquid chromatography.

Main Results:

  • The MOF-coated vial enabled analysis with only 50 μL of solvent and a total operation time of 12.5 minutes.
  • A simple cleaning protocol effectively resolved protein pore-blocking and allowed for over 50 reuses of the vial.
  • Sensitive detection of seven bisphenols was achieved, with limits of detection as low as 0.10 μg/L and good precision (RSD < 15%).

Conclusions:

  • A miniaturized MOF-coated vial device was successfully developed for analyzing complex saliva samples.
  • The device demonstrates reusability and overcomes protein clogging challenges, maintaining analytical performance.
  • This approach signifies a notable advancement in bioanalytical sample preparation, particularly for integrated miniaturized devices.