Related Experiment Video
Updated: May 25, 2025
Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
Published on: September 6, 2017
[Sequence Analysis and Confirmation of an HLA Null Allele Generated by a Base Insertion]
Zhan-Rou Quan1, Yan-Ping Zhong1, Liu-Mei He1
1Institute of Transfusion Medicine, Shenzhen Blood Center, Shenzhen 518040, Guangdong Province, China.
Objective:
To confirm the sequence of a null allele HLA-C*08:127N produced by a base insertion.
Methods:
PCR sequence-specific oligonucleotide probe (SSOP) and PCR sequence-based typing (SBT) were used for HLA routine detection, which discovered abnormal sequence maps of HLA-C in one acute myeloid leukemia patient. The sequence of the above loci was confirmed by next generation sequencing (NGS) technology.
Results:
The SSOP typing result showed that HLA-C locus was C*03:04, C*08:01, while the sequence was suspected to be inserted or deleted in exon 3 by SBT, and finally confirmed by NGS as C*03:04, C*08:127N.
Conclusion:
When base insertion produces HLA null alleles, SBT analysis software cannot provide correct results, but NGS technology can more intuitively obtain accurate HLA typing results.
