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Phallus indusiatus Extracts Promoted MCF-7 Apoptosis Under TNFα-induced Tumor Microenvironment by Attenuating

Saranya Sedtananun1,2, Penporn Sujiwattanarat3, My Ha Hoang Tra4

  • 1Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of b Technology North Bangkok, Bangkok 10800, Thailand.

Asian Pacific Journal of Cancer Prevention : APJCP
|March 1, 2025
PubMed
Abstract

Insights

Phallus indusiatus extracts show anti-inflammatory properties and enhance the effectiveness of lapatinib in breast cancer cells by promoting apoptosis and reducing inflammatory signaling pathways.

Area of Science:

  • Mycology
  • Pharmacology
  • Cancer Biology

Background:

  • Breast cancer is often associated with an inflammatory microenvironment that can lead to drug resistance.
  • Phallus indusiatus, a medicinal mushroom, has potential bioactive compounds that may possess therapeutic properties.

Purpose of the Study:

  • To investigate the anti-inflammatory and anticancer effects of Phallus indusiatus extracts.
  • To assess their ability to modulate inflammatory conditions and enhance the efficacy of lapatinib in breast cancer cells.

Main Methods:

  • Water extracts of Phallus indusiatus were prepared and tested for anti-inflammatory effects on RAW246.7 cells.
  • Cytotoxicity and proliferation were evaluated in MCF-7 and MCF-10A breast cell lines.
  • The impact of extracts on lapatinib-resistant MCF-7 cells under inflammatory conditions (TNFα-induced) was assessed, including effects on cell viability, apoptosis, and NF-kappaB signaling.

Main Results:

  • Phallus indusiatus extracts exhibited anti-inflammatory activity by reducing nitric oxide (NO) production.
  • Extracts did not show cytotoxicity in breast cell lines but increased proliferation in MCF-10A cells.
  • Under inflammatory conditions, extracts slightly reduced MCF-7 cell viability and sensitized lapatinib-induced cytotoxicity by increasing apoptosis.
  • Co-treatment attenuated AKT and NF-kappaB activation.

Conclusions:

  • Phallus indusiatus extracts possess anti-inflammatory and anticancer properties.
  • These extracts can sensitize breast cancer cells to lapatinib, particularly in inflammatory microenvironments.
  • Phallus indusiatus may serve as a beneficial supplementary food for breast cancer patients.
Keywords:
ApoptosisInflammationPhallus indusiatusbreast cancermicroenvironment

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