Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Development and Performance Analysis of High-K Spacer-Induced Strained Si/SiGe Channel-Based Gate All Around FET for Thermal Effects.

Nanomaterials (Basel, Switzerland)·2025

Same author

Comparative analysis of the structure and crystallin composition of the lenses of freshwater fish and gastropods with respect to their vision.

Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology·2025

Same author

Cardiovascular events in patients with psoriasis and psoriatic arthritis treated with JAK/TYK inhibitors: A systematic review and meta-analysis.

Journal of the European Academy of Dermatology and Venereology : JEADV·2024

Same author

Edge-Terminated AlGaN/GaN/AlGaN Multi-Quantum Well Impact Avalanche Transit Time Sources for Terahertz Wave Generation.

Nanomaterials (Basel, Switzerland)·2024

Same author

Assessment of Safety and Prophylactic Efficacy of the EpiVacCorona Peptide Vaccine for COVID-19 Prevention (Phase III).

Vaccines·2023

Same author

The Rectifying Contact of Hydrated Different Size YSZ Nanoparticles for Advanced Electronics.

Nanomaterials (Basel, Switzerland)·2022

Search research articles

Related Experiment Video

Updated: May 2, 2026

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

Sers-based methodology for nanomolar methotrexate concentration detection for clinics.

Elizaveta A Demishkevich¹, Svetlana A Stefanova¹, Andrey Y Zyubin¹

¹Immanuel Kant Baltic Federal University, 14 A. Nevskogo str., Kaliningrad, Russia 236041.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy

|March 1, 2025

Summary

This study introduces a fast and affordable method using silver nanoparticles for detecting methotrexate (MTX) in blood serum. The technique offers a promising alternative to traditional clinical analysis methods.

Keywords:

Methotrexate Nanoparticles Plasmon resonance SERS Spectrum Surface-enhanced Raman spectroscopy TDM Therapeutic drug monitoring

More Related Videos

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

Published on: October 25, 2013

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

Published on: July 14, 2017

Related Experiment Videos

Last Updated: May 2, 2026

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

Published on: October 25, 2013

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

Published on: July 14, 2017

Area of Science:

Nanotechnology
Analytical Chemistry
Biomedical Engineering

Background:

Methotrexate (MTX) is a crucial drug in chemotherapy.
Accurate and rapid detection of MTX in biological samples is vital for patient treatment.
Current detection methods like HPLC can be time-consuming and expensive.

Purpose of the Study:

To develop a novel, rapid, and reliable method for detecting methotrexate (MTX) in human blood serum.
To utilize truncated triangular silver nanoparticles (AgNP) for enhanced detection sensitivity.
To provide a cost-effective alternative to existing clinical diagnostic techniques.

Main Methods:

Synthesis of truncated triangular silver nanoparticles (AgNP) on quartz glass substrates.
Application of Surface-Enhanced Raman Spectroscopy (SERS) for MTX detection.
Testing the method with pure MTX, MTX with metabolites, and patient serum samples.

Main Results:

Detection of pure MTX down to 10^-9 M concentration.
Successful detection of MTX in human plasma samples at clinical concentrations up to 10^-6 M.
Demonstrated reliability and speed of the developed SERS-based method.

Conclusions:

The developed AgNP-based SERS method provides a rapid, sensitive, and cost-effective approach for MTX detection in serum.
This technique shows potential as a valuable alternative to conventional methods like HPLC in clinical settings.
The study highlights the utility of nanomaterials in advancing diagnostic capabilities for therapeutic drug monitoring.