Related Experiment Video
Updated: May 24, 2025

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
Published on: May 24, 2017
"Repair and fold" DNA nanotweezers for measuring DNA alkylation repair mediated by ALKBH
Fengze Jiang1, Cui Zhang1, Xiangnan Wang1,2
1State Key Laboratory of Chemo/Bio-Sensing, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. tomwu@hnu.edu.cn.
Abstract:
We report a novel DNA lesion-modified nanotweezer that underwent conformational switching upon ALKBH enzyme-mediated lesion repair. We demonstrated that the DNA lesion-modified nanotweezer enabled specific and rapid monitoring of ALKBH2 and ALKBH3 activities and screening of inhibitors. Importantly, this strategy allowed evaluating ALKBH2-associated drug resistance in cells.
Related Concept Videos
Nucleotide Excision Repair
Fixing Double-strand Breaks
Base Excision Repair
The first step of...

