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Label-Free Multiphoton Microscopy for Diagnosis of Psoriasis
Liqin Zheng1, Liuzhi Wu2, Wenwen Zhong3
1Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China.
Abstract:
Psoriasis is a global problem that significantly affects patients both physically and mentally. Accurate and rapid detection of psoriasis is crucial for diagnosis and treatment. Herein, we utilized multiphoton microscopy (MPM) to rapidly image psoriasis in imiquimod-induced mouse and human psoriatic samples. Our results showed that MPM accurately monitored the thickening of stratum corneum and epidermis in psoriatic tissues, similar to traditional H&E staining. Notably, we observed an increased alignment and number of collagen fibers in both mouse models and human psoriatic samples. Furthermore, a decrease in the proportionate area, length, width, and cross-linking gap of collagen fibers, as well as an increase in cross-linking density was also obtained in mouse psoriatic models. These findings suggest that collagen can be a biomarker for evaluating the progress of psoriasis. Overall, our study presents a rapid diagnostic method for psoriasis using MPM.
Insights
Multiphoton microscopy (MPM) offers a rapid diagnostic method for psoriasis. This technique accurately images psoriatic tissue changes and identifies collagen fiber alterations, suggesting collagen as a potential biomarker for disease progression.
Area of Science:
- Dermatology
- Biomedical Imaging
- Optical Microscopy
Background:
- Psoriasis is a prevalent global skin condition with significant physical and psychological impacts.
- Timely and precise diagnosis of psoriasis is essential for effective patient management and treatment.
- Current diagnostic methods can be time-consuming, necessitating faster imaging techniques.
Purpose of the Study:
- To investigate the utility of multiphoton microscopy (MPM) for rapid imaging and detection of psoriasis.
- To analyze microstructural changes in psoriatic tissues, specifically stratum corneum, epidermis, and collagen fibers.
- To evaluate the potential of collagen as a biomarker for assessing psoriasis progression.
Main Methods:
- Utilized multiphoton microscopy (MPM) to image imiquimod-induced mouse models and human psoriatic skin samples.
- Compared MPM imaging findings with traditional Hematoxylin and Eosin (H&E) staining.
- Quantified changes in collagen fiber characteristics, including alignment, number, area, length, width, cross-linking gap, and density.
Main Results:
- MPM accurately monitored the thickening of the stratum corneum and epidermis in psoriatic tissues, consistent with H&E staining.
- Observed increased alignment and number of collagen fibers in both mouse and human psoriatic samples.
- Detected decreased proportionate area, length, width, and increased cross-linking density of collagen fibers in mouse models.
Conclusions:
- Multiphoton microscopy provides a rapid and accurate method for diagnosing psoriasis.
- Alterations in collagen fiber structure (alignment, density, and dimensions) serve as potential biomarkers for psoriasis.
- MPM imaging of collagen changes offers a promising avenue for evaluating psoriasis progression and severity.
