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Protocol for designing a peptide-based multi-epitope vaccine targeting monkeypox using reverse vaccine technology
Amit Kumar1, Garima Nagar2, Prithwik Bhowmik2
1National Institute of Immunology, New Delhi, India.
STAR Protocols
|March 7, 2025
Summary
This study introduces a novel peptide-based vaccine protocol for monkeypox virus (MPXV) using immunoinformatics. The open-source approach ensures robust immune defense against rapidly evolving pathogens.
Area of Science:
- Vaccinology
- Immunoinformatics
- Computational Biology
Background:
- Reverse vaccine technology accelerates vaccine development for emerging infectious diseases.
- Monkeypox virus (MPXV) poses a significant public health threat requiring novel vaccine strategies.
Purpose of the Study:
- To present an open-source protocol for developing a peptide-based multi-epitope vaccine against MPXV.
- To leverage immunoinformatics for designing effective and stable vaccine candidates.
Main Methods:
- In silico evaluation of physicochemical properties and allergenicity.
- Validation of pattern recognition receptor (PRR)-binding affinity and major histocompatibility complex (MHC) I/II presentation.
- Molecular dynamics (MD) simulations to confirm immune receptor interactions and vaccine stability.
Main Results:
- The protocol outlines a systematic approach for designing MPXV vaccine candidates.
- Computational analyses predict favorable vaccine properties and stability.
- Demonstrated feasibility of using immunoinformatics for rapid vaccine development.
Conclusions:
- The presented protocol offers a viable strategy for creating peptide-based vaccines against MPXV.
- This open-source approach can be adapted for other rapidly evolving pathogens.
- Advancements in immunoinformatics are crucial for strengthening global immune defense.
