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Updated: May 23, 2025

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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
Published on: August 6, 2014
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Construction of a dual-color fluorescent light-up biosensor based on sequential coding for label-free and sensitive
Jinping Hu1, Jie Zhang1, Juan Hu1
1School of Chemistry and Chemical Engineering, State Key Laboratory of Digital Medical Engineering, Southeast University, Nanjing 211189, China. hujuan@seu.edu.cn.
Abstract:
We construct a dual-color light-up biosensor for label-free and sensitive detection of multiple piRNAs. This biosensor exhibits high sensitivity with a limit of detection of 3.19 aM for piRNA-36026 and 4.71 aM for piRNA-823. Moreover, it can accurately measure cellular piRNA levels with single-cell sensitivity, and discriminate breast cancer tissues from healthy tissues.

