Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.9K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

173
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
173
Radical Reactivity: Overview01:11

Radical Reactivity: Overview

2.0K
Radicals, the highly reactive species, gain stability by undergoing three different reactions. The first reaction involves a radical-radical coupling, in which a radical combines with another radical, forming a spin‐paired molecule. The second reaction is between a radical and a spin‐paired molecule, generating a new radical and a new spin‐paired molecule. The third reaction is radical decomposition in a unimolecular reaction, forming a new radical and a spin‐paired...
2.0K
Cationic Chain-Growth Polymerization: Mechanism00:57

Cationic Chain-Growth Polymerization: Mechanism

2.2K
The cationic polymerization mechanism consists of three steps: initiation, propagation, and termination. In the initiation step of the polymerization process, the π bond of a monomer gets protonated by the Lewis acid catalyst, which is formed from boron trifluoride and water. The protonation of the π bond generates a carbocation stabilized by the electron‐donating group. In the propagation step, the π bond of the second monomer acts as a nucleophile and attacks the...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photoinduced Disproportionation Enables Oxidative Addition of Aryl Iodides at a Gallium(I) Center.

Journal of the American Chemical Society·2026
Same author

AgBF<sub>4</sub>-catalyzed insertion of unactivated alkynes into C-F bonds of acyl fluorides.

Chemical science·2026
Same author

Synthesis, Structure, and Reactivity of a Neutral Indium(I) Complex Bearing a Phenalenyl-Based N,N-Bidentate Ligand.

Inorganic chemistry·2026
Same author

Nickel-catalyzed decarbonylative addition of acylsilanes across alkynes <i>via</i> the cleavage of a carbon-silicon bond.

Chemical science·2025
Same author

Synthesis of Phenylenediamines via (4 + 1 + 1) Photocycloaddition of 1,3-Dienes and Isocyanides Enabled by a Gallium(I)/(III) Redox: The Key Role of a Phenalenyl-Based Ligand.

Journal of the American Chemical Society·2025
Same author

Cobalt-Catalyzed Decarbonylation of Aromatic Acylsilanes via the Cleavage of Carbon-Silicon Bonds.

Chemistry, an Asian journal·2025

Related Experiment Video

Updated: May 22, 2025

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.1K

Unimolecular Fragment Coupling and Single Carbon Atom Doping as Tools for Structural Reprogramming.

Hayato Fujimoto1,2, Mamoru Tobisu1,2

  • 1Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.

Accounts of Chemical Research
|March 13, 2025
PubMed
Summary

This study introduces unimolecular fragment coupling (UFC) and single carbon atom doping (SCAD) for molecular skeletal editing. These methods enable efficient construction of complex molecules through precise atom manipulation, advancing organic synthesis.

More Related Videos

Versatile CO2 Transformations into Complex Products: A One-pot Two-step Strategy
07:36

Versatile CO2 Transformations into Complex Products: A One-pot Two-step Strategy

Published on: November 9, 2019

7.9K
Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function
05:57

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function

Published on: April 26, 2024

308

Related Experiment Videos

Last Updated: May 22, 2025

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.1K
Versatile CO2 Transformations into Complex Products: A One-pot Two-step Strategy
07:36

Versatile CO2 Transformations into Complex Products: A One-pot Two-step Strategy

Published on: November 9, 2019

7.9K
Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function
05:57

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function

Published on: April 26, 2024

308

Area of Science:

  • Organic Synthesis
  • Medicinal Chemistry
  • Material Science

Background:

  • Precise atom deletion or insertion is crucial for molecular construction and diversification.
  • Existing methods often rely on functional group interconversions or intermolecular couplings.
  • Need for novel strategies that offer enhanced selectivity and atom economy.

Purpose of the Study:

  • To introduce and demonstrate the utility of unimolecular fragment coupling (UFC) and single carbon atom doping (SCAD) for skeletal reprogramming.
  • To showcase UFC and SCAD as powerful tools for efficient molecular modifications beyond conventional approaches.
  • To highlight the potential of these methods in accelerating the synthesis of complex molecules and novel architectures.

Main Methods:

  • Unimolecular Fragment Coupling (UFC): Involves intramolecular elimination and recombination of molecular fragments.
  • Nickel(0)/N-heterocyclic carbene (NHC)-mediated reactions for decarbonylation and decarboxylation.
  • Single Carbon Atom Doping (SCAD): Insertion of a single carbon atom into a molecular skeleton using NHC catalysts.

Main Results:

  • Developed nickel-catalyzed UFC for synthesizing biaryls from diaryl ketones, amides, and acylsilanes.
  • Achieved catalytic decarboxylative and deisocyanative UFC of aryl carbamates and amides, respectively.
  • Demonstrated SCAD for one-step synthesis of lactams from acyclic precursors via four-bond formation at a single carbon center.

Conclusions:

  • UFC and SCAD represent a new paradigm in skeletal editing, offering precise control over molecular frameworks.
  • These methodologies provide efficient, selective, and atom-economical alternatives to traditional synthetic routes.
  • The developed strategies significantly expand the synthetic chemist's toolbox for complex molecule synthesis and discovery.