Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nano-Silver-Selenium Liquid Dressing Facilitates Treatment of Monkeypox and Prevention of Viral Transmission in a Surrogate Mouse Model.

Exploration (Beijing, China)·2026

Same author

Facet-Dependent Water Inhibition of Alkanol Dehydration on TiO<sub>2</sub> via Distinct Water-Alkanol Complexes.

Angewandte Chemie (International ed. in English)·2026

Same author

Characterization Standard for <i>In-situ</i> Cryo-electron Tomography.

bioRxiv : the preprint server for biology·2026

Same author

Structural characterization of kappa-opioid receptor dimer in complex with two G proteins.

Nature communications·2026

Same author

AreTomoLive: automated reconstruction of comprehensively corrected and denoised cryo-electron tomograms in real time and at high throughput.

Nature methods·2026

Same author

Microglia-Targeted Biomimetic Tetrahedral Framework Nucleic Acid Nanovesicles for Synergistic Treatment of Sepsis-Associated Encephalopathy.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Search research articles

Related Experiment Video

Updated: May 22, 2025

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

Deconvolution to restore cryo-EM maps with anisotropic resolution.

Yifan Cheng¹, Junrui Li², Yifei Chen²

¹HHMI/University of California San Francisco.

Research Square

|March 17, 2025

Summary

This summary is machine-generated.

Single particle cryogenic electron microscopy (cryo-EM) can suffer from preferred orientations, causing anisotropic resolution. A new deconvolution method, AR-Decon, computationally improves 3D map quality from such datasets.

More Related Videos

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

Related Experiment Videos

Last Updated: May 22, 2025

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

Area of Science:

Structural biology
Biophysics
Biochemistry

Background:

Single particle cryogenic electron microscopy (cryo-EM) is a powerful tool for determining high-resolution structures.
Accurate 3D structure determination relies on randomly oriented particles.
Preferential particle orientations lead to anisotropic resolution, limiting structural accuracy.

Purpose of the Study:

To develop a computational method to address anisotropic resolution in cryo-EM data.
To improve the quality of 3D maps reconstructed from datasets with preferred orientations.

Main Methods:

Established a deconvolution approach named AR-Decon.
Tested and validated AR-Decon with synthetic and experimental cryo-EM datasets.
Compared AR-Decon's performance against alternative machine-learning based methods.

Main Results:

AR-Decon computationally improves the quality of 3D maps with anisotropic resolutions.
The method effectively corrects for artifacts caused by preferred particle orientations.
Validation demonstrated the robustness of AR-Decon on diverse datasets.

Conclusions:

AR-Decon offers a viable computational solution for enhancing cryo-EM map quality.
This method can help overcome preferred orientation challenges in structural biology.
AR-Decon contributes to more accurate and reliable structural determination using cryo-EM.