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Updated: May 21, 2025

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
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Mapping Selenium Nanoparticles Distribution Inside Cells through Confocal Raman Microspectroscopy.

Davide Redolfi-Bristol1,2, Kenta Yamamoto3, Wenliang Zhu1

  • 1Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, Japan.

ACS Applied Materials & Interfaces
|March 18, 2025
PubMed
Summary
This summary is machine-generated.

Biocompatible selenium nanoparticles (SeNPs) show promise for biomedical imaging and therapy. Confocal Raman microspectroscopy successfully mapped SeNPs within human dermal fibroblast cells, revealing cellular uptake without significant toxicity.

Keywords:
2D mapping3D imagingRaman microspectroscopycytotoxicityselenium nanoparticles

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Area of Science:

  • Nanotechnology
  • Cell Biology
  • Spectroscopy

Background:

  • Selenium nanoparticles (SeNPs) possess valuable biomedical properties, including antimicrobial, anticancer, and anti-inflammatory effects.
  • Understanding nanoparticle-cell interactions is crucial for developing effective biomedical applications.

Purpose of the Study:

  • To synthesize biocompatible SeNPs and investigate their distribution within human dermal fibroblast (HDF) cells using confocal Raman microspectroscopy.
  • To assess the cytotoxicity of SeNPs on HDF cells.
  • To demonstrate the utility of Raman microspectroscopy for imaging SeNPs within cells and studying their dynamic uptake.

Main Methods:

  • Synthesis of biocompatible SeNPs.
  • Confocal Raman microspectroscopy for 2D and 3D mapping of SeNPs within HDF cells.
  • Cell viability assays to evaluate SeNP cytotoxicity.

Main Results:

  • SeNPs were successfully synthesized and demonstrated a unique Raman signature for precise imaging.
  • SeNPs showed minimal cytotoxicity, with adverse effects only observed at high concentrations and prolonged exposure.
  • Three-dimensional Raman mapping provided novel insights into SeNP cellular penetration.
  • Two-dimensional mapping confirmed dynamic SeNP uptake and internalization over time at sublethal concentrations.

Conclusions:

  • Confocal Raman microspectroscopy is an effective technique for visualizing SeNP distribution and dynamics within cells.
  • Biocompatible SeNPs hold potential for biomedical imaging and therapeutic applications.
  • This study offers a new method for investigating nanoparticle-cell interactions.