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The Correlation between GSK3β and miR-140 and its Effect on the Pathogenesis of Asthma

Ting Yang1, Chang Xu1, Niu Ding1

  • 1Department of Respiratory Medicine, Hunan Children's Hospital, 410000 Changsha, Hunan, China.

Discovery Medicine
|March 21, 2025
PubMed
Abstract

Insights

MicroRNA-140 (miR-140) plays a crucial role in asthma by reducing airway inflammation and apoptosis. This study reveals that miR-140 targets glycogen synthase kinase 3 beta (GSK3β), offering a potential therapeutic pathway for asthma.

Area of Science:

  • Molecular Biology
  • Immunology
  • Respiratory Medicine

Background:

  • MicroRNAs (miRNAs) are implicated in the progression of asthma.
  • Investigating the specific role of miR-140 in asthma pathogenesis is essential.

Purpose of the Study:

  • To elucidate the functional role of miR-140 in asthma.
  • To determine the molecular mechanism by which miR-140 influences asthma progression.

Main Methods:

  • Utilized a murine model of asthma and an in vitro cell model (16HBE cells treated with TGF-β1).
  • Assessed miR-140 levels via RT-qPCR, histopathological changes using HE and PAS staining, and inflammatory markers via ELISA.
  • Evaluated protein expression of Bcl-2, GSK3β, cleaved caspase-3, and BAX using Western blotting and IHC.
  • Confirmed the interaction between GSK3β and miR-140 using RIP and dual-luciferase reporter assays.

Main Results:

  • Asthma models showed reduced miR-140 and elevated GSK3β.
  • Overexpression of miR-140 attenuated airway inflammation and apoptosis in asthma models.
  • A direct targeting relationship was confirmed between miR-140 and GSK3β.
  • GSK3β upregulation counteracted the beneficial effects of miR-140 overexpression in vitro.

Conclusions:

  • miR-140 acts as a protective factor in asthma.
  • The mechanism involves targeting and regulating GSK3β, thereby mitigating airway inflammation and apoptosis.
  • Modulating miR-140 and GSK3β presents a potential therapeutic strategy for asthma.
Keywords:
GSK3βairway inflammationasthmamiR-140

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