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Related Experiment Video

Updated: May 20, 2025

Silicon Nanowires and Optical Stimulation for Investigations of Intra- and Intercellular Electrical Coupling

Silicon Nanowires and Optical Stimulation for Investigations of Intra- and Intercellular Electrical Coupling

Published on: January 28, 2021

Vertically Aligned Nanowires for Longitudinal Intracellular Sampling.

David Eun Reynolds¹, Yoon Ho Roh^2,3, Uday Chintapula²

¹Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.

|March 27, 2025

Summary

This summary is machine-generated.

This study introduces a novel nanowire platform for tracking messenger RNA (mRNA) in living cells over time without cell lysis. This method enables dynamic cellular analysis and drug response monitoring in the same cells, advancing longitudinal omics research.

Keywords:

intracellular sampling longitudinal profiling nanowires temporal transcriptomics

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Area of Science:

Cellular biology
Nanotechnology
Molecular profiling

Background:

Omics technologies offer insights into cellular functions but often require cell lysis, hindering longitudinal studies.
Existing methods for live-cell longitudinal profiling face challenges in throughput and genetic manipulation.
Nanomaterials, especially nanowires, present opportunities for high-throughput, low-cost cellular analysis due to their unique properties.

Purpose of the Study:

To develop a nanowire-based platform for longitudinal messenger RNA (mRNA) profiling in living cells.
To enable minimal disruption mRNA extraction from living cells for repeated analysis.
To demonstrate the platform's capability in tracking dynamic cellular changes over time.

Main Methods:

Utilized vertically aligned nickel nanowire arrays for efficient mRNA extraction.
Implemented a nanowire platform for non-destructive, longitudinal profiling of individual living cells.
Monitored enhanced green fluorescent protein (eGFP) expression dynamics and transcriptomic shifts in response to drug treatments.

Main Results:

Successfully performed longitudinal mRNA profiling in living cells using the nanowire platform.
Demonstrated the ability to track changes in gene expression (e.g., eGFP) in the same cells over extended periods.
Showcased the platform's utility in analyzing cellular responses to drug stimuli dynamically.

Conclusions:

The developed nanowire platform facilitates high-throughput, longitudinal mRNA analysis in living cells with minimal disruption.
This technology overcomes limitations of traditional omics methods, enabling dynamic cellular studies.
The platform holds significant potential for advancing research in cell biology, drug discovery, and personalized medicine.