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Aspirin Eugenol Ester Modulates the Hypothalamus Transcriptome in Broilers Under High Stocking Density
Xiaodie Zhao1,2, Yi Zhang1,2, Dongying Bai1,2
1Department of Animal Physiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China.
Animals : an Open Access Journal From MDPI
|March 28, 2025
Summary
High-density broiler farming can hinder growth, but aspirin eugenol ester (AEE) supplementation improves performance. This study investigated how AEE affects the broiler hypothalamus transcriptome and growth under high-density conditions.
Area of Science:
- Animal Science
- Poultry Nutrition
- Molecular Biology
Background:
- High-density (HD) stocking in broiler production can lead to competition, negatively impacting growth compared to normal density (ND).
- Aspirin eugenol ester (AEE) has shown potential to improve growth performance in HD broilers, but the underlying mechanisms, particularly concerning hypothalamus-regulated feeding behavior, remain unclear.
Purpose of the Study:
- To investigate the effects of dietary AEE supplementation on the hypothalamic transcriptome of broilers under HD conditions.
- To correlate observed transcriptomic changes with broiler production performance metrics in HD environments.
Main Methods:
- Four treatment groups were established: Normal Density (ND), High Density (HD), ND with AEE, and HD with AEE, using Arbor Acres broilers.
- Broiler growth performance (body weight, feed intake, gain, feed conversion) was monitored until day 28.
- Hypothalamus samples were collected on day 28 for transcriptome analysis.
Main Results:
- Broilers in the HD group exhibited significantly reduced body weight compared to the ND group (p < 0.01).
- AEE supplementation significantly improved average daily feed intake, average daily gain, and feed conversion ratio in HD broilers from days 22 to 28 (p < 0.05).
- Transcriptome analysis revealed 20 commonly enriched signaling pathways between ND vs. HD and HD vs. HD-AEE groups, identifying potential genes involved in CNS development and feed intake regulation.
Conclusions:
- Dietary AEE supplementation effectively ameliorates the negative effects of high-density housing on broiler growth performance.
- AEE influences hypothalamic gene expression patterns, offering new insights into the regulation of feeding behavior and growth in broilers under stressful environmental conditions.
