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Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
Redox-Regulated Cell Response Mechanisms to Nitrosyl Iron Complex with N-Ethylthiourea in Human Mesenchymal Stem
A A Balakina1, V I Amozova2, T S Stupina2
1Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry, Russian Academy of Sciences, Chernogolovka, Moscow Region, Russia. balakina@icp.ac.ru.
Abstract:
We studied the processes of cell death and intracellular accumulation of free radicals, as well as activity of redox-sensitive transcription factors Nrf2 and NF-κB under the action of nitrosyl iron complex with N-ethylthiourea (ETU) on a model of human mesenchymal stem cells in vitro. ETU complex exhibits a pronounced prooxidant and cytotoxic effects accompanied by enhanced intracellular accumulation of nitrogen monoxide, induction of apoptotic cell death, activation of Nrf2/HO-1 pathways, and suppression of proinflammatory NF-κB-mediated reactions. A significant role of redox-dependent cellular processes in the mechanisms of the cytotoxic effect of nitrosyl iron complexes is shown.
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