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Wavefront correction with image-based interferometric focus sensing in two-photon microscopy.

Ruiwen Yang^1,2, Yanlong Yang¹, Tengfei Wu¹

¹State Key Laboratory of Ultrafast Optical Science and Technology, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.

Nanophotonics (Berlin, Germany)

|March 31, 2025

Summary

This summary is machine-generated.

Adaptive optics corrects wavefront distortions for better imaging. Image-based interferometric focus sensing (IBIFS) improves deep tissue imaging by progressively correcting aberrations across the entire field of view.

Keywords:

adaptive optics image-based metric interferometric focus sensing two-photon microscopy

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Last Updated: May 17, 2025

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Published on: April 9, 2014

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Area of Science:

Biomedical Optics
Microscopy
Adaptive Optics

Background:

Adaptive optics (AO) enhances image quality by correcting wavefront distortions.
Interferometric focus sensing (IFS) is an AO technique effective for deep tissue imaging but typically uses a single sample point.
Complex aberrations limit imaging depth and clarity in biological samples.

Purpose of the Study:

To introduce and validate an image-based interferometric focus sensing (IBIFS) method.
To enable wavefront correction over an extended field of view in microscopy.
To improve the stability and effectiveness of adaptive optics for deep tissue imaging.

Main Methods:

Developed an image-based interferometric focus sensing (IBIFS) technique.
Implemented IBIFS within a sample conjugate adaptive optics configuration.
Utilized image quality metrics for progressive wavefront estimation and correction.
Experimentally validated using two-photon microscopy on fluorescent beads and mouse brain slices.

Main Results:

Demonstrated effective wavefront correction across a large field of view.
Achieved more stable optimization results compared to region-of-interest based methods.
Successfully applied IBIFS to complex biological samples like mouse brain slices.

Conclusions:

IBIFS offers a robust and effective solution for aberration correction in deep tissue imaging.
The proposed method expands the field of view for adaptive optics microscopy.
IBIFS provides enhanced stability and performance for advanced imaging applications.