High-Content Imaging-Based Screening for Anticancer Compounds in Zebrafish Xenografts

Larval zebrafish xenografts are a vertebrate model system suited for screening small compounds towards their anticancer effects. The small size and transparency of zebrafish larvae allow for phenotypic screening in 96-well format. Due to the achievable throughput in a living vertebrate organism, zebrafish xenografts promise to bridge the gap between in vitro screening and testing compounds in mouse xenografts. Here, we describe a workflow for establishing xenografts in zebrafish embryos/larvae for subsequent automated high-content imaging-based screening of small compounds.