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Updated: May 16, 2025

Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish

Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish

Published on: April 10, 2020

High-Content Imaging-Based Screening for Anticancer Compounds in Zebrafish Xenografts.

Caterina Sturtzel¹, Sarah Grissenberger¹, Andrea Wenninger-Weinzierl¹

¹St. Anna Children's Cancer Research Institute, Vienna, Austria.

Methods in Molecular Biology (Clifton, N.J.)

|March 31, 2025

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Larval zebrafish xenografts offer a high-throughput vertebrate model for screening anticancer compounds. This method bridges in vitro and mouse xenograft studies using automated imaging.

Area of Science:

Comparative medicine
Developmental biology
Pharmacology

Background:

Zebrafish larvae are transparent and small, facilitating phenotypic screening.
Current methods for anticancer compound screening have limitations in throughput and biological relevance.
Bridging in vitro and in vivo models is crucial for drug discovery.

Purpose of the Study:

To establish a workflow for xenografting in zebrafish embryos and larvae.
To enable automated high-content imaging-based screening of small compounds.
To validate zebrafish xenografts as a vertebrate model for anticancer drug screening.

Main Methods:

Development of a standardized xenografting protocol in zebrafish embryos/larvae.
Implementation of automated high-content imaging for phenotypic analysis.

Keywords:

High-content imaging Larval zebrafish xenografts Small compound screening Tumor cells

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Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

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Preclinical Assessment of the Bioactivity of the Anticancer Coumarin OT48 by Spheroids, Colony Formation Assays, and Zebrafish Xenografts

Preclinical Assessment of the Bioactivity of the Anticancer Coumarin OT48 by Spheroids, Colony Formation Assays, and Zebrafish Xenografts

Published on: June 26, 2018

Related Experiment Videos

Last Updated: May 16, 2025

Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish

Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish

Published on: April 10, 2020

Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

Published on: June 19, 2021

Preclinical Assessment of the Bioactivity of the Anticancer Coumarin OT48 by Spheroids, Colony Formation Assays, and Zebrafish Xenografts

Preclinical Assessment of the Bioactivity of the Anticancer Coumarin OT48 by Spheroids, Colony Formation Assays, and Zebrafish Xenografts

Published on: June 26, 2018

Optimization of compound screening in a 96-well format using the zebrafish model.

Main Results:

Successful establishment of a reproducible xenograft model in larval zebrafish.
Demonstration of automated, high-content imaging for efficient data acquisition.
Validation of the model's suitability for phenotypic screening of small compounds.

Conclusions:

Zebrafish xenografts provide a powerful, high-throughput vertebrate system for anticancer compound screening.
This model effectively bridges the gap between early-stage in vitro assays and later-stage in vivo studies.
The described workflow enables efficient and automated drug discovery in a living organism.