Topogami: Catenating DNA Origami

DNA origami is a powerful tool for designing and building functional nanoscale machines. There are practical limitations on the size of a single origami meaning that methods to combine multiple DNA origamis into single functional units would be useful. Catenation of DNA origami structures would provide arguably the most stable linkage method, but true catenation requires linking of the DNA origami scaffold strands. This is achieved in the DNA Topogami method, outlined here, which employs a resolvase to produce the scaffold catenanes, which can then be folded into the required structures by staple strands.