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Related Concept Videos

DNA Replication02:40

DNA Replication

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DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
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During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
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The Replisome03:01

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
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The DNA Replication Fork01:02

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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
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DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
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DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

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Topogami: Catenating DNA Origami.

Gerrit Wilkens1, Piotr Stepien2, Ahmed Shaukat2

  • 1Université de Montpellier, Centre de Biochimie Structurale, CNRS, INSERM, Montpellier, France.

Methods in Molecular Biology (Clifton, N.J.)
|April 2, 2025
PubMed
Summary
This summary is machine-generated.

The DNA Topogami method enables stable linkage of DNA origami structures by catenating their scaffold strands using a resolvase enzyme. This technique allows for the creation of larger, more complex nanoscale machines from multiple DNA origami units.

Keywords:
DNA catenanesDNA origamiDNA ringsTn3 resolvase

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Area of Science:

  • Nanotechnology
  • Molecular Biology
  • Biotechnology

Background:

  • DNA origami is a versatile method for constructing nanoscale machines.
  • Limitations exist in the size of individual DNA origami structures.
  • Combining multiple DNA origami units is necessary for larger functional constructs.

Purpose of the Study:

  • To present the DNA Topogami method for linking DNA origami structures.
  • To enable the creation of larger, stable nanoscale assemblies.
  • To overcome size limitations of single DNA origami units.

Main Methods:

  • The DNA Topogami method utilizes a resolvase enzyme.
  • Scaffold strands of DNA origami structures are catenated.
  • Staple strands are used to fold the catenated scaffolds into desired structures.

Main Results:

  • Achieved true catenation by linking DNA origami scaffold strands.
  • Demonstrated a method for stable linkage of multiple DNA origami units.
  • Enabled the formation of larger, integrated DNA origami constructs.

Conclusions:

  • The DNA Topogami method provides a stable linkage for DNA origami structures.
  • This technique facilitates the construction of complex, multi-unit nanoscale machines.
  • DNA Topogami overcomes previous size limitations in DNA origami assembly.