Advances and challenges in polyhydroxyalkanoates (PHA) production using Halomonas species: A review

Plastic waste pollution is one of the major threats to sustainable development. Biodegradable polymers and biopolymers such as polyhydroxyalkanoates (PHAs) offer suitable alternatives for replacing synthetic plastics. PHAs are produced by diverse bacteria species and archaea as storage compounds for utilization as carbon and energy sources. Halomonas species have emerged as attractive microbial cell factories for biosynthesis of PHAs due to their metabolic versality, ability to valorize diverse feedstock materials, and tolerance to high salinity and pH that allows fermentation in contamination-resistant conditions. In recent years, there has been great attention to the use of Halomonas species in PHA biosynthesis and genetic engineering efforts for enhanced production. This article provides a discussion of the current state of knowledge on production of polyhydroxyalkanoates by Halomonas species. It includes an overview of PHA biosynthesis mechanisms, fermentation strategies, production with cheap substrates, exploitation of open and unsterile conditions, co-production of PHAs and other products, and advances genetic engineering efforts.