Efficient genetic transformation and genome editing via an Agrobacterium-mediated in commercial oat (Avena sativa L.) cultivars
Kun Shi1, Weihong Huang2, Mengxin Zhu3
1College of Grassland Science and Technology, China Agricultural University, Beijing, 100193, China.
Journal of Integrative Plant Biology
|April 7, 2025
Abstract:
An optimized Agrobacterium-mediated transformation protocol for immature and mature oat embryos increased transformation efficiencies and the number of transformable cultivars and enabled highly efficient CRISPR/Cas9 and CRISPR/Cas12i genome editing to accelerate oat biotechnology breeding.
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