Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

2.2K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.2K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

2.3K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.3K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

2.4K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.4K
Disassembly of Intermediate Filaments01:35

Disassembly of Intermediate Filaments

2.0K
Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
2.0K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

3.0K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.0K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

2.6K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A rice calmodulin-like protein OsCML27 participates in drought tolerance by fine-tuning OsRbohB-mediated ROS signaling.

Science advances·2026
Same author

Dynamics of the β-cardiac myosin auto-inhibited state explain cardiomyopathy pathogenesis.

Nature communications·2026
Same author

FAD-Linked Oxidoreductase Protein 1 (FLO1) Coordinates Grain Development and Drought Tolerance in Rice.

Plants (Basel, Switzerland)·2026
Same author

XsMYB58 regulates XsLACS8 to promote the accumulation of seed oil in Yellowhorn.

The Plant journal : for cell and molecular biology·2026
Same author

Structural mechanism of RECQ1 helicase in unfolding G-quadruplexes compared with duplex DNA.

Nucleic acids research·2025
Same author

XsLTPG31 Confers Leaf Cuticular Wax Deposition and Drought Resistance in Yellowhorn.

Plant, cell & environment·2025

Related Experiment Video

Updated: May 15, 2025

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner
09:02

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner

Published on: December 10, 2015

7.3K

NAL1 forms a molecular cage to regulate FZP phase separation.

Ling-Yun Huang1,2, Ting-Ting Wang1, Peng-Tao Shi1,3

  • 1Department of Biotechnology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.

Proceedings of the National Academy of Sciences of the United States of America
|April 9, 2025
PubMed
Summary

Narrow Leaf 1 (NAL1) protein acts as a molecular cage, regulating the phase separation of FRIZZY PANICLE (FZP) to enhance plant gene transcription and crop productivity.

Keywords:
NAL1molecular cagephase separationproteasestransactivation

More Related Videos

FtsZ Polymerization Assays: Simple Protocols and Considerations
12:04

FtsZ Polymerization Assays: Simple Protocols and Considerations

Published on: November 16, 2013

15.2K
In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
08:56

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Published on: May 5, 2020

5.8K

Related Experiment Videos

Last Updated: May 15, 2025

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner
09:02

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner

Published on: December 10, 2015

7.3K
FtsZ Polymerization Assays: Simple Protocols and Considerations
12:04

FtsZ Polymerization Assays: Simple Protocols and Considerations

Published on: November 16, 2013

15.2K
In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
08:56

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Published on: May 5, 2020

5.8K

Area of Science:

  • Plant Biology
  • Molecular Mechanisms
  • Agronomy

Background:

  • Narrow Leaf 1 (NAL1) is crucial for rice morphology and agronomic traits.
  • The molecular mechanisms of NAL1's diverse functions remain largely unknown.

Purpose of the Study:

  • To elucidate the structural basis of NAL1 function.
  • To understand how NAL1 regulates the phase separation of its substrate, FRIZZY PANICLE (FZP).

Main Methods:

  • Comprehensive structural analysis of NAL1 proteins.
  • In vitro and in vivo investigation of FZP phase separation.
  • Assessment of NAL1's role in regulating FZP condensation and transcriptional activity.

Main Results:

  • NAL1 forms a hexameric molecular cage with specific channels for substrate discrimination.
  • FZP undergoes phase separation, forming molecular condensates.
  • NAL1 fine-tunes FZP condensation and enhances transcriptional activity via proteolytic regulation.

Conclusions:

  • NAL1's structure enables precise regulation of FZP phase separation and transcriptional activity.
  • This mechanism offers insights into NAL1's multifaceted roles in plant development.
  • Findings support rational breeding strategies for improved crop productivity.