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Sensitive Ratiometric Luminescent FRET Probe for the Detection of Riboflavin.

Wei Liu1, Wei Yao1, Andrei S Potapov2

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New metal-organic frameworks (MOFs) act as sensitive sensors for riboflavin (RF). Ho-MOF offers excellent anti-interference, rapid detection, and practical application in real samples, demonstrating high potential for RF monitoring.

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Area of Science:

  • Materials Chemistry
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Riboflavin (RF) is a vital nutrient, and its accurate quantification is crucial for health and food quality.
  • Developing selective and sensitive detection methods for RF remains a significant challenge in analytical chemistry.
  • Metal-organic frameworks (MOFs) offer tunable properties for advanced sensing applications.

Purpose of the Study:

  • To synthesize novel isomorphic 2D MOFs for luminescent sensing of riboflavin.
  • To investigate the sensing performance, including selectivity, sensitivity, and stability, of the synthesized MOFs.
  • To explore the practical applicability of the developed MOF-based sensor in real-life samples.

Main Methods:

  • Solvothermal synthesis of two isomorphic 2D MOFs: Ho-MOF and Er-MOF.
  • Utilizing Förster resonance energy transfer (FRET) for ratio luminescent sensing of riboflavin.
  • Evaluating sensor performance through anti-interference tests, pH stability, response time, and cyclic detection.

Main Results:

  • Both Ho-MOF and Er-MOF exhibited excellent anti-interference capabilities and low detection limits for RF (as low as 1.72 nM for Ho-MOF).
  • Ho-MOF demonstrated superior performance with enhanced anti-interference, a low detection limit (1.72 nM), excellent pH stability (4-12), and rapid response (<50 s).
  • Ho-MOF showed practical applicability in real samples like urine and serum, with detection limits of 5.26 nM and 27.27 nM, respectively, and high recovery rates in VB2 tablets.

Conclusions:

  • The synthesized Ho-MOF and Er-MOF are effective luminescent sensors for riboflavin based on the FRET mechanism.
  • Ho-MOF exhibits outstanding sensing properties, making it a promising candidate for practical, selective, and sensitive riboflavin detection.
  • This study highlights the potential of MOFs in developing advanced analytical tools for monitoring vital compounds in diverse environments.